the relationship between ventricular dilatation, neuropathological and neurobehavioural changes in hydrocephalic rats心室扩张之间的关系,神经病理学和脑积水的大鼠神经行为改变的.pdfVIP

Olopade et al. Fluids and Barriers of the CNS 2012, 9:19 FLUIDS AND BARRIERS /content/9/1/19 OF THE CNS RESEARCH Open Access The relationship between ventricular dilatation, neuropathological and neurobehavioural changes in hydrocephalic rats 1 1,2* 3 Funmilayo Eniola Olopade , Matthew Temitayo Shokunbi and Anna-Leena Sirén Abstract Background: The motor and cognitive deficits observed in hydrocephalus are thought to be due to axonal damage within the periventricular white matter. This study was carried out to investigate the relationship between ventricular size, cellular changes in brain, and neurobehavioural deficits in rats with experimental hydrocephalus. Methods: Hydrocephalus was induced in three-week old rats by intracisternal injection of kaolin. Behavioural and motor function were tested four weeks after hydrocephalus induction and correlated to ventricular enlargement which was classified into mild, moderate or severe. Gross brain morphology, routine histology and immunohistochemistry for oligodendrocytes (CNPase), microglia (Iba-1) and astrocytes (GFAP) were performed to assess the cellular changes. Results: Decreases in open field activity and forelimb grip strength in hydrocephalus correlated with the degree of ventriculomegaly. Learning in Morris water maze was significantly impaired in hydrocephalic rats. Gradual stretching of the ependymal layer, thinning of the corpus callosum, extracellular oedema and reduced cortical thickness were obser