the role of actin isoforms in somatic embryogenesis in norway spruce肌动蛋白异型体的作用在挪威云杉体细胞胚胎发生.pdfVIP

Schwarzerová et al. BMC Plant Biology 2010, 10:89 /1471-2229/10/89 R E S E A R C H A R T I C L E Open Access Research article The role of actin isoforms in somatic embryogenesis in Norway spruce †2 †1 1 1 2 Kateřina Schwarzerová†1, Zuzana Vondráková , Lukáš Fischer , Petra Boříková , Erica Bellinvia , Kateřina Eliášová , 1 1,3 2 1 Lenka Havelková , Jindřiška Fišerová , Martin Vágner and Zdenek Opatrný* Abstract Background: Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs. Results: Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells a