the t7-related pseudomonas putida phage φ15 displays virion-associated biofilm degradation properties的t7-related假单胞菌putida噬菌体φ15显示virion-associated生物膜降解特性.pdfVIP

The T7-Related Pseudomonas putida Phage Q15 Displays Virion-Associated Biofilm Degradation Properties 1 1 1 1 2 Anneleen Cornelissen , Pieter-Jan Ceyssens , Jeroen T’Syen , Helena Van Praet , Jean-Paul Noben , 3 3 1 1 Olga V. Shaburova , Victor N. Krylov , Guido Volckaert , Rob Lavigne * 1 Laboratory of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium, 2 School of Life Sciences, Biomedical Research Institute and Transnational University Limburg, Hasselt University, Diepenbeek, Belgium, 3 State Institute for Genetics of Industrial Microorganisms, Moscow, Russia Abstract Formation of a protected biofilm environment is recognized as one of the major causes of the increasing antibiotic resistance development and emphasizes the need to develop alternative antibacterial strategies, like phage therapy. This study investigates the in vitro degradation of single-species Pseudomonas putida biofilms, PpG1 and RD5PR2, by the novel phage Q15, a ‘T7-like virus’ with a virion-associated exopolysaccharide (EPS) depolymerase. Phage Q15 forms plaques surrounded by growing opaque halo zones, indicative for EPS degradation, on seven out of 53 P. putida strains. The absence of haloes on infection resistant strains suggests that the EPS probably act as a primary bacterial receptor for phage infection. Independent of bacterial strain or biofilm age, a time and dose dependent response of Q15-mediated biofilm degradation was observed with generally a maximum biofilm degradation 8 h after addition of the higher phage doses (104 and 106 pfu) and resistance development after 24 h. Biofilm age