痰培养的采集（Collection of sputum cultures）.doc

痰培养的采集（Collection of sputum cultures） Collection and interpretation of sputum culture Infectious pathogenic diagnosis and clinical microbiology laboratory - improving the quality of antimicrobial susceptibility test in bacterial culture Clinicians send qualified specimens - accurate interpretation of reports Main points of microbial specimen collection and transportation General principles - before the use of antibiotics, collection, collection, timely inspection, preferably in 0.5h, shall not exceed 2h. Specimens submitted for delay or treatment should be stored at 4 degrees centigrade. The specimens should be processed in 24h. - avoid contamination by normal flora - sufficient quantity of specimens Special culture cup Correct collection of sputum samples Sputum samples are collected early in the morning, because there is a large amount of sputum and a large amount of bacteria . Take samples under the guidance of a doctor or nurse. Ask the patient to gargle three times to remove the superficial bacteria. To instruct the patient for deep cough to obtain specimens of the lower respiratory tract (rather than the secretions of the saliva and nasopharynx) in a sterile container Be careful: Expectoration can not be anaerobic culture!!! The correct collection of patients with coma or inability to cough and sputum If the patient is unconscious or unable to cough, assist the patient to take the proper position, knock back to loosen the sputum, and then draw the suction device, The sputum collecting device is connected with the sputum collecting device in the middle of the suction tube, and the sputum is sucked into the sputum collection apparatus according to the sputum aspiration method. Suction tube aspiration can avoid contamination of the normal flora of the mouth. It is mainly used for the infection of anaerobic bacteria in the lungs and the immune damage And the atypical pneumonia, but its side effects are large and the patients are not easy to accept. It has rarely b