第一篇细胞工程的技术基础（First, the technical basis of cellular engineering）.docVIP

第一篇细胞工程的技术基础（First, the technical basis of cellular engineering） First, the technical basis of cellular engineering Chapter 1 facilities and basic conditions of cell culture The second chapter, cleaning and disinfection (preparation before training) The third chapter is the basic method of cell culture Chapter 1 facilities and basic conditions of cell culture I. setting of cellular engineering laboratory 1 setting up animal cell engineering laboratory Aseptic operating room: changing room, buffer room, operating room Culture observation room Preparation room Storeroom Cleaning and sterilizing room 2 plant cell engineering laboratory setting In addition to the same setting as the animal cell engineering laboratory, there are special requirements, such as light, plantlet cultivation and transplant domestication. Two, commonly used instruments and equipment Over clean bench Inverted microscope and phase contrast microscope Drying box Water purification unit Refrigerator (4 DEG C, -20 DEG C, -70 DEG C) Pressure steam sterilizer Cell counting plate and electronic cell counter Filtering sterilizing device Centrifuge Pipette Centrifuge tube Pipette balance General or conventional instruments and equipment Animal cell engineering laboratory basic equipment 1 incubator Carbon dioxide incubator provides constant CO2 (5%) to stabilize pH, temperature: mammals 35 pH7.2-7.4 -37 C, birds 38.5 DEG C, gas phase: O2, CO2, C 2 micro manipulator 3 cell fusion apparatus 4 cell frozen storage (programmable freezer and liquid nitrogen tank -196 DEG C) 5 culture containers, various culture flasks, culture plates, porous culture plates (6, 24, 96) 6 surgical instruments and so on Plant cell engineering laboratory basic equipment Light incubator Artificial climate room Table Culture flask Three. Biosafety of the laboratory Depending on the degree of tightness, biosafety is divided into four levels, i.e.: P1, P2, P3, P4 (protect) General cell culture according to the standard of Biosafety Le