胸腺肽a1对白血病细胞吲哚胺2%2c3-加双氧酶蛋白表达及免疫调节作用实验的研究.pdfVIP

The experiment of IDO protein of leukemia Cells cultured with Ta1 Abstract Part Ⅰ:Ta1 reduce the IDO of leukemia induced by IFN-γ Objective:Discuss the influence and mechanism that Ta1 downregulation IDO oxygen enzyme of leukemia.Methods: 1.The drug induction of cells:Trials are divided into IFN-γ/Ta1 inhibit IDO expression and IFN-γ/Ta1 cut IDO express group,Each of different concentration and time of treatment.2.Cracking protein extraction.3.The Western Blot test each of protein.4.ECL dyeing, multiple color fluorescence imaging camera for every purpose extract corresponding of pixels than analysis of the semi.Results:1.Not join IFN-γ, IDOmRNA is visible shows a faint,IDO not seen clearly on molecularly imprinted ,IDO shows on molecularly imprinted bands increased.2. After IFN-γ induced, then add the Ta1 role, IDO strip with Ta1 concentration and function significantly weakened, the map income pixels also account.3.IDO channel with same Ta1 concentration and the growth of the action time significantly weakened,Tip Ta1 can weaken IFN-γ to IDO entrainment, express reduced.Conclusion: 1.IDO a low level in leukemia .And the degradation of tryptophan cause T proliferation and apoptosis thwarted increased, leading to the immune tolerance,So hint IDO may close relationship with immune.2.IFN-γ can significantly raise IDO .After Join IFN-γ, IDO express clearly enhance, tip IFN-γ may promote the leukemia cells immune to escape, leading to a large number of tumor cell growth,And IFN-γ is typical of Th1 cells factor type, with immune adjustment and antitumor function,So in IFN-γ used in the treatment of patients with leukemia, should be vigilant of the formation mechanism