纤维素酶对枯草期牦牛消化率和采食量影响.pdfVIP

Effect of cellulase on forage intake and digestibility of yaks grazing on withered grassland Jiang Guixia(animal nutrition) Directed by Xue Bai Abstract The activity of cellulase from Ningxia Xiasheng Enzyme Corporation Limited was surveyed using the substrates of carboxymethyl cellulose(CMC)and filter paper, respectively,with dinitrosalicylic acid method (DNS)as chromogenic fluid. The results were 362 U/g for CMC and 580 U/g for filter paper. Different substrates led to different results of enzyme activity, therefore, substrate must be clearified when illustrate enzyme activity. An in vitro study was used to investigate the mechanism by which cellulase improve feed conversion. The method was conducted as a completely randomized design in three replications with treatments arranged as a 5 (incubation times) ×5(cellulase doses)factorial design. The dose of enzyme were 0‰, 1‰, 2‰, 3‰, and 4‰ of wheat stalk(DM basic).Soluble sugar and soluble substance were measured after terminating the incubation at 6h, 12h, 18h, 24h, 36h, and they increased in different extent with dose of cellulase and the incubation time. The optimal dose of this kind cellulase was 3‰, and the optimal time for the enzyme degradation was 24h. Soluble sugar and soluble substance were 7.5% and 28.91% at 24h with 3‰ dose of this kind cellulase. 20 healthy and castrated grazing yaks, 2 years old, were divided into four groups randomly, a