免疫色谱法及发光免疫分析法测量血清降钙素原.docVIP

免疫色谱法及发光免疫分析法测量血清降钙素原 　【摘要】 目的 目前临床上常用的检测血清降钙素原（Procalcitonin PCT）的方法：半定量免疫色谱法（PCT-Q）和定量发光免疫分析法（PCT-L），我们的实验目的是比较这两种方法的一致性，更好地为临床诊断、治疗服务。方法 73份人血清标本，分别采用PCT-Q和PCT-L两种方法检测PCT含量。PCT-Q检测结果将样本浓度分为四个级别，即：＜0.5 ng/ml、≥0.5＜2 ng/ml、≥2＜10 ng/ml和≥10 ng/ml。一致性用Cohens统计获得。结果 65.8 %样本PCT-Q与PCT-L的检测结果在同一级别，27.4 %样本检测结果上或下相差一个级别，一致性检验k=0.506，符合尚好。结论 虽然利用 PCT-Q检测血清PCT浓度快速并简单易行，但PCT-Q与PCT-L的检测结果并不总是一致的，对于有疑问或要求监测治疗的病例，PCT-L仍是首选的检测方法。 【关键词】 降钙素原；半定量；免疫色谱法；发光免疫分析法 Comparison of serum procalcitonin test between immunochromatograph and immunoluminometry 【Abstract】 Objective The coherence comparison of serum procalcitonin (PCT) between two methods: the semi -quantitative solid phase immunoassay（PCT-Q）and the quantitative luminometric assay（PCT-L）. Methods Seventy three samples were analyzed with PCT-Q. Results obtained were categorized into 4 concentration ranges : ＜0.5 ng/ml、≥0.5＜2ng/ml、≥2＜10 ng/ml和≥10ng/ml. In parallel, serum procalcitonin concentrations were measured with PCT-Q as a standard . The data of PCT-Q and PCT-L were analysied with Cohen?s k as a measure of concordance.Results 65.8% of samples were consistently categorized according to the results of PCT-Q and PCT-L . 27.4% of samples were categorized within the next higher or lower concentrations category in the methods . Cohen?s kvalue is 0.506. Conclusion PCT-Q was a quick and easy handle methods for serum PCT measurement. However the results of PCT-Q were not always identical comparison to the PCT-L. So PCT-L was a good choice for questionable cases and the monitoring of treatment. 【Key words】 procalcitonin(PCT);immunochromatographic test;immunoluminometric assay 降钙素原（Procalcitonin PCT）作为一种新的严重细菌感染早期诊断指标，在国外已广泛应用于ICU，并取得了良好效果。PCT是一个具有创新意义的诊断指标，具有三大突出优点，一是可用于疾病的早期诊断：严重全身性细菌感染2～3hPCT浓度开始升高，24～48h达到峰值。二是用于疾病的鉴别诊断， PCT是一种新的高敏感性和特异性细菌感染与非细菌感染鉴别诊断的指标［1，2］。三是它可用于感染病情的监测：PCT浓度与细菌感染严重程度呈正相关关系，随着感染的控制和病情的缓解，PCT浓度下降。目前临床常用检测PCT含量的方法有半定量免疫色谱法（Semiquantitative immunochromatographic test PCT-Q）和定量发光免疫分析法（Quantitative immunoluminomet