党参多糖对糖尿病小鼠胰岛素抵抗改善作用.docVIP

党参多糖对糖尿病小鼠胰岛素抵抗改善作用 　【摘要】 目的观察党参多糖对糖尿病小鼠、胰岛素抵抗小鼠的作用。方法观察党参多糖300，200，100 mg/kg对正常小鼠血糖的影响；采用一次性腹腔注射四氧嘧啶（Alloxan,ALX,200 mg/kg）建立糖尿病小鼠模型，观察党参多糖300，200，100 mg/kg 对糖尿病小鼠血糖、血清胰岛素水平的影响，并测定血清SOD，MDA的含量；皮下注射氢化可的松琥珀酸钠（HCSS,70 mg/kg）诱导胰岛素抵抗小鼠模型，通过腹腔注射胰岛素后小鼠血糖变化观察党参多糖300，200，100 mg/kg对小鼠胰岛素敏感性的影响。结果党参多糖对正常小鼠血糖无影响(Pgt;0.05)；与模型对照组比较，党参多糖各剂量组显著降低四氧嘧啶诱导的糖尿病小鼠血糖和血清胰岛素水平（Plt;0.05或Plt;0.01），提高糖尿病小鼠血清SOD的活性，减少MDA产生(Plt;0.05或Plt;0.01)；对氢化可的松琥珀酸钠诱导的小鼠胰岛素抵抗有显著的改善作用(Plt;0.05或Plt;0.01)。结论党参多糖能降低糖尿病小鼠的血糖，改善小鼠的胰岛素抵抗。 【关键词】 党参多糖； 糖尿病； 胰岛素抵抗 Abstract：ObjectiveTo investigate the effects of polysaccharides from Radix Codonopsis on insulin resistance in diabetic mice.MethodsFasting blood glucose was observed in normal mice by ig polysaccharides from Radix Codonopsis (300,200,100 mg/kg). Meanwhile, fasting blood glucose and serum insulin were measured to evaluate the hypoglycemic effects of polysaccharides from Radix Codonopsis (300,200,100 mg/kg) on diabetic model mice that induced by ip alloxan 200 mg/kg, the activity of SOD and the content of MDA in serum were also determined. In addition, the variety of blood glucose after ip insulin was determined to measure the influence of Polysaccharides from Radix Codonopsis (300,200,100 mg/kg) on insulin sensitivity in insulin resistance model mice induced by sc HCSS (70 mg/kg). ResultsPolysaccharides from Radix Codonopsis had no significantl effect on fasting blood glucose of normal mice (Pgt;0.05). Compared with the model control group, polysaccharides from Radix Codonopsis effectively decreased the fasting blood glucose and the insulin in serum (Plt;0.05 or Plt;0.01), enhanced the SOD activity, reduced the MDA content in serum (Plt;0.05 or Plt;0.01); At the same time, Polysaccharides from Radix Codonopsis improved the insulin resistance in insulin resistance model mice. All the results were dose-dependent. ConclusionPolysaccharides from Radix Codonopsis possesses a significant hypoglycemic effect in diabetic mice by improving the insulin resistance.