白介素2抑制T细胞特异性免疫应答机制探究.docVIP

白介素2抑制T细胞特异性免疫应答机制探究 　 作者：张腾龙,谢彦晖,王缨,林果为 【摘要】 　　本研究查明白介素2（IL2）对静息T细胞抗原特异性免疫应答的作用，探讨IL2对静息T细胞中细胞因子信号传导抑制蛋白(suppressor of cytokine signaling 3，SOCS3 )表达的影响，及其与抗原特异性免疫应答的关系。用IL2（50 U/ml）预处理静息DO11.10 T细胞，洗涤去除IL2后用3HTdR掺入法检测静息DO11.10 T细胞针对OVA323-329抗原(ovalbumin，OVA，卵清蛋白)的特异性增殖；用IL2（50 U/ml）刺激静息DO11.10 T细胞，然后用荧光实时定量PCR法检测SOCS3在刺激后不同时间的表达变化；用OVA323-329抗原活化静息DO11.10 T细胞，然后检测SOCS3在抗原活化后不同时间的表达变化。结果表明：静息DO11.10 T细胞经IL2刺激后抗原特异性增殖能力减弱；静息DO11.10 T细胞经IL2刺激后SOCS3表达上调，在刺激4小时后表达即明显上调，6小时后达到最高峰；静息DO11.10 T细胞经OVA323-329抗原活化后SOCS3表达明显下调，在活化后第2天降至最低，4天后基本恢复正常。结论： IL2在一定条件下可抑制静息T细胞抗原特异性免疫应答，而且这种抑制作用可能与SOCS3表达上调有关。 【关键词】 IL2；DO11.10 T细胞；SOCS3；OVA323-329抗原 　 Abstract The study was aimed to investigate the effect of IL2 on the acquired immune response of naive T cells，and to explore the influence of IL2 on suppressor of cytokine signaling 3 (SOCS3) expression of naive T cells, as well as to elucidate the role of SOCS3 on antigen specific immune response in vitro. Naive DO11.10 T cells were prestimulated with IL2 (50 U/ml), and stimulated with OVA323-329 antigen after removing IL2, then the proliferation of naive DO11.10 T cells was detected. Naive DO11.10 T cells were stimulated with IL2 (50 U/ml), and SOCS3 expression was detected by realtime PCR. Naive DO11.10 T cells were stimulated with OVA323-329 antigen, and SOCS3 expression was detected by means of 3HTdR. The results showed that after IL2 prestimulation, the proliferation of naive DO11.10 T cells decreased significantly when stimulated with OVA323-329 antigen; SOCS3 expression of naive DO11.10 T cells was upregulated significantly after IL2 stimulation, the upregulation began obviously at 4 hours and reached peak at 6 hours. SOCS3 expression on naive DO11.10 T cells was downregulated markedly after OVA323-329 antigen stimulation, the expression level of SOCS3 was lowest on day 2 and returned to normal on day 4 after stimulation. It is concluded that the antigen specific immune response of naive