磷酸化AKT、AKT2、PTEN在NSCLC中表达及意义.docVIP

磷酸化AKT、AKT2、PTEN在NSCLC中表达及意义 　 作者：苗丽君,孙振涛,王静,吴秋歌,吴逸明, 吴拥军 【摘要】 目的 探讨AKT信号转导通路中有关蛋白磷酸化AKT（pAKT）、AKT2及PTEN在非小细胞肺癌(NSCLC)中的表达、相关性及意义。方法 应用免疫组化的方法，检测80例NSCLC组织及35例非肿瘤性肺组织标本中pAKT、AKT2、PTEN的表达，并与临床病理因素进行相关性分析。结果 pAKT、AKT2在NSCLC中高表达，阳性率分别为78.8%、91.3%，显著高于非肿瘤性肺组织中的0、5.7%(Plt;0.05)。PTEN在NSCLC中低表达，阳性率47.5%，显著低于非肿瘤性肺组织中的 94.3% (Plt;0.05)。pAKT的表达与患者年龄、性别、组织学类型及分化程度、TNM分期、淋巴结转移无关(Pgt;0.05)。AKT2的表达与淋巴结转移有关，在淋巴结转移组的阳性表达率100%显著高于非淋巴结转移组表达率80.6%（Plt;0.05）。PTEN表达与淋巴结转移、组织的分化程度有关(Plt;0.05)。AKT2与pAKT呈正相关（rs =0.596，Plt;0.01）。PTEN表达与pAKT（rs =-0.240，Plt;0.05）、AKT2（rs=-0.326，Plt;0.01）呈负相关。结论 在NSCLC中存在AKT的活化，PTEN表达的丢失与AKT的活化有关，AKT2是PTEN的调节形式之一。 【关键词】 非小细胞肺癌；pAKT；AKT2； PTEN 　　The Expression and Clinical Significance of phosphoAKT, AKT2 and PTEN Proteins in Human Nonsmall Cell Lung Cancer 　　Abstract：Objective To investigate the expression, clinical significance and relationship of phosphoAKT (pAKT), AKT2 and PTEN in human nonsmall cell lung cancer (NSCLC) tissue.Methods The expression of pAKT ,AKT2 and PTEN in 80 cases of NSCLC and 35 cases of nocancerous lung disease were assessed by immunohistochemistry, and their correlations with clinicopathologic factors were statistically analyzed.Results The positive rate of pAKT, AKT2 was 78.8%、91.3% in NSCLC and 0%、5.7% (Plt;0.05) in nocancerous lung disease. But the positive rate of PTEN (47.5%) was significantly lower than that of PTEN (94.3%) in nocancerous lung disease (Plt;0.05). The expression of pAKT didn’t relate to age, sex, histological subtype and tumor differentiation, lymph node metastasis and TNM stages(Pgt;0.05). The positive rate of AKT2 in the group with lymph node metastasis (100.0%) was significantly higher than that of AKT2 in the group without lymph node metastasis（80.6%）(Plt;0.05). The expression of PTEN correlated with lymph node metastasis and differentiation of NSCLC (Plt;0.05). In addition, Positive correlations were observed between the expression of AKT2 and pAKT in NSCLC tissues(Plt;0.05). The express