色素上皮源性因子对缺血—再灌注视网膜神经节细胞保护作用.docVIP

色素上皮源性因子对缺血—再灌注视网膜神经节细胞保护作用 　 作者：李文化　杨永升　李海燕　马涛 【摘要】 　　目的：研究色素上皮源性因子（pigment epithelium derived factor，PEDF）对高眼压诱导的大鼠视网膜缺血—再灌注后视网膜神经节细胞的保护作用。方法：经眼角膜进行前房平衡盐水（BSS）灌注，维持眼内压110mmHg，以阻止视网膜正常血液灌注。60min后取出灌注针头，恢复视网膜正常血流，从而建立大鼠视网膜缺血—再灌注模型。实验分为正常非缺血组和视网膜缺血—再灌注组，后者又分为生理盐水注射对照组和PEDF注射实验组，再灌注模型建立后立即向实验组大鼠玻璃体腔内注射0.2g/L PEDF 2μL。实验对照组用同样方法注射等量生理盐水。分别于注射后2d和7d进行眼球摘除，对视网膜进行光学显微镜形态学观察和Fas原位杂交免疫学分析，探讨PEDF对缺血—再灌注视网膜神经节细胞的保护作用。结果：缺血—再灌注2d时生理盐水注射组和PEDF注射组视网膜神经节细胞明显少于正常对照组(P ＜0.01)和(P ＜0.05)，PEDF注射组视网膜神经节细胞数较生理盐水注射组明显较多，相比有显著性差异（P ＜0.05）；视网膜神经节细胞计数再灌注7d后结果与2d时类似。再灌注2d生理盐水注射组Fas阳性染色细胞比PEDF注射组明显较多（P ＜0.05），生理盐水组比PEDF注射组阳性细胞百分率明显较高(P ＜0.01)；再灌注7d时两组Fas阳性细胞计数无明显差异。结论：视网膜缺血—再灌注后即刻行玻璃体腔内PEDF注射可以改善视网膜神经节细胞的损伤并有一定保护作用。 【关键词】 视网膜缺血—再灌注损伤　色素上皮源性因子　凋亡　Fas 　　Pigment epithelium derived factor as a neuroprotective agent against Ischemic-reperfusion induced retinal gonglion cell injury Abstract AIM: To determine whether PEDF is neuroprotective for retinal neurons that are exposed to transient pressure induced ischemia-reperfusion. METHODS: Transient retinal ischemia was produced by increasing the intraocular pressure for 60 minutes in SD rats eyes，immediately after reperfusion，PEDF was injected intravitreally into the experimental eyes. Injury was evaluated morphologically and by counting the number of the retinal ganglion cells(RGC) and number of Fas staining cells. RESULTS: Morphologically，RGCs appeared turbulence and capsular changes and morphmetrically， the number of RGCs were significantly protected at 2 and 7 days in PEDF injected eyes. Fas in situ Hybridization showed the number of positive stained cells in PEDF injected eyes was obviously less than the vehicle injected eyes. CONCLUSION: PEDF injection immediately after ischemic-reperfusion can ameliorate retinal gonglion cells injury. It may be useful in preventing neuronal damage in the retinal gonglion cell layer induced by retinal ischemia. · KEYWORDS: retina ischemic-reperfusion; pigment epithelium derived factor; apoptosis; Fas 　　0引言 视