苍耳子中酚酸类化合物及不同品种及居群苍耳子中总酚酸含量测定.docVIP

苍耳子中酚酸类化合物及不同品种及居群苍耳子中总酚酸含量测定 　 作者：韩婷，李慧梁，胡园，张巧艳，黄宝康，郑汉臣，Khalid RAHMAN，秦路平 【关键词】 苍耳子 ［摘要］ 目的：研究中药苍耳子（Fructus Xanthii）中的化学成分；测定不同居群和不同品种苍耳属植物果实中的总酚酸含量以评价其品质。方法：运用各种层析方法分离纯化化合物，以绿原酸作对照，用紫外分光光度法测定总酚酸的含量。结果：（1）从苍耳子中分离到6个咖啡酰奎宁酸类化合物，以及咖啡酸和阿魏酸；（2）从中国29个居群采集到的样品，总酚酸含量为0.31％～1.44%。在采集到2个品种及1个变种的苍耳属植物中，近无刺苍耳X. sibiricum var. subinerme样品的总酚酸含量较其他两种都偏低。苍耳X. sibiricum样品中，从上海采集的3号苍耳子样品总酚酸含量最高，达1.44%，而从江西新建采集的12号样品含量最低，仅0.38%。结论：5O咖啡酰奎宁酸，1，4-二O咖啡酰奎宁酸和4，5二O咖啡酰奎宁酸是从苍耳属植物中首次分离获得。不同品种和不同居群苍耳子药材中，总酚酸含量的差异较为显著。若以总酚酸含量作为指标评价苍耳子药材的品质，则认为上海和福建三明产苍耳子为优质种群。 　　［关键词］ 苍耳子; 酚酸; 咖啡酰奎宁酸; 紫外分光光度法; 化学结构鉴定; 居群; 含量测定; 质量评价 　　Phenolic acids in Fructus Xanthii and determination of contents of total phenolic acids in different species and populations of Xanthium in China 　　　ABSTRACT Objective: To study the chemical constituents of Fructus Xanthii and to determine the contents of total phenolic acids (TPA) in fruits of Xanthium from different populations for evaluating the quality of them. Methods: Components in Fructus Xanthii were isolated and purified by various column chromatographies and the contents of TPA were determined by ultraviolet spectrophotometry with chlorogenic acid (CHA) as reference substance. Results: Six caffeoylquinic acids along with caffeic acid and ferulic acid were isolated from Fructus Xanthii. The contents of TPA of the samples collected from 29 populations in China varied from 0.31% to 1.44%. Among the samples originated from two species and 1 variety of Xanthium, the contents of TPA in X. sibiricum var. subinerme samples with an average of 0.36% were relatively lower than those in other 2 species. While the content of TPA in Sample 3 collected from Shanghai was 1.44% and the highest among all the samples, and that in Sample 12 from Xinjian of Jiangxi Province was 0.38% and the lowest among the X. sibiricum samples. Conclusion: 5Ocaffeoylquinic acid, 1,4diOcaffeoylquinic acid and 4,5diOcaffeoylquinic acid were isolated from Xanthium plant for the first time. The difference of