苦瓜蛋白抑制肿瘤基质金属蛋白酶活性及表达.docVIP

苦瓜蛋白抑制肿瘤基质金属蛋白酶活性及表达 　作者：熊术道，尹丽慧，李景荣，叶爱芳，章圣辉，温博贵 【摘要】 　　［目的］ 探讨苦瓜蛋白对H22荷瘤小鼠基质金属蛋白酶表达及其活性的影响。［方法］ 将清洁级ICR雄性小鼠随机分成5组，建立肝癌H22移植瘤模型，分为阴性对照组、高剂量组(T3)、中剂量组(T2)、低剂量组(T1)及阳性对照组（CTX,环磷酰胺处理组）。在普通饲料饲喂的基础上采用腹腔注射给药法分别予阴性对照组生理盐水；高、中、低剂量组分别给以不同剂量苦瓜蛋白；阳性对照组予环磷酰胺。观察苦瓜蛋白对抑瘤率的影响，明胶酶谱法检测荷瘤小鼠血清基质金属蛋白酶MMP-2、MMP-9的活性及其变化。［结果］ 苦瓜蛋白组低、中、高剂量组及阳性对照组抑瘤率分别为52.05%、66.89%、85.81％及65.10％；各苦瓜蛋白干预组与阴性对照组相比，MMP-2、MMP-9的活性降低，酶原表达下调(Plt;0.01)，上述作用与苦瓜蛋白剂量相关。［结论］ 苦瓜蛋白具有抑制基质金属蛋白酶表达及其活性的作用，其抗肿瘤作用可能与苦瓜蛋白具有抑制MMPs的活性及其表达有关。 【关键词】 苦瓜蛋白　移植性肿瘤　抗肿瘤作用　基质金属蛋白酶 　　Abstract: ［Purpose］ To explore effect of Momordin on expression and activity of matrix metalloprotease in murine transplanted tumor(H22). ［Methods］ Male mice of ICR species were randomly divided into 5 groups and then constructed the transplantation tumor model of liver cancer H22. The negative control group were treated by normal saline; T1, T2 and T3 group by Momordin with low, middle and high dose respectively and positive control group by cytoxan (CTX group). All the mice were feeded ordinarily with intraperitoneal drugs administration. Tumor inhibitive rate of Momordin was measured. Activity of MMP-2 and MMP-9 in serum was detected by Gelatin Zymography. ［Results］ Tumor inhibition rate in low, middle and high dose of Momordin and CTX group were 52.05%, 66.89%, 85.81% and 65.10%, respectively. The activity of MMP-2 and MMP-9 significantly decreased in all Momordin treated groups compared to the negative control group (Plt;0.01). The above function correlated to the dose of Momordin. ［Conclusion］ Momordin plays a role of inhibitory effect on the expression and activity of matrix metalloprotease and its anti-tumor activity may be involved in regulating the expression and activity of matrix metalloprotease. Key words: Momordin; transplanted tumor; anti-tumor activity; matrix metalloprotease. 苦瓜(momordica charantia) 属葫芦科植物，中医认为其具有消暑涤热、明目、解毒之功效。苦瓜蛋白(momordin)是从苦瓜籽中分离出来的一种分子量约为30kD的碱性糖蛋白质，属于Ⅰ型核糖体失活蛋白(ribosome inhibiting protein，RIP)。近年来研究表明RIP具有明显的抗肿瘤、抗病毒作用