SO2暴露对运动大鼠心肌间质胶原纤维的影响及其可能-环境科学研究.docVIP

S02污染对运动大鼠心功能的影响：基于AT1R介导的心肌胶原纤维形态结构重塑的研究 陈 平1 胡艳茹1 刘晓莉1* 1 师范大学体育与运动学院，北京摘要：SO2污染环境下运动对大鼠的影响，从心脏局部肾素血管紧张素系统（renin-angiotensin system，RAS）核心成员血管紧张素angiotensin Ⅱ，AngⅡ）Ⅰ型受体（AT1R）采用放射免疫技术（ELISA）免疫组织化学胃酶酸解法心肌局部ρ(Ang)、AT1R蛋白表达羟脯氨酸含量胶原容积分数。结果表明：⑴单纯运动组（EG）大鼠主动脉收缩压、左室内压峰值、左室内压最大上升速率/下降速率显著升高（P0.01），舒张压、AT1R蛋白表达显著降低（P0.01），心肌羟脯氨酸含量、心肌胶原浓度、血管周围胶原面积心肌胶原容积分数及ρ(Ang)有升高趋势（P0.01）；⑵单纯SO2污染组（SRG）大鼠左室末期舒张压显著升高（P0.01），左室内压最大上升速率/下降速率显著降低（P0.01）；心肌局部羟脯氨酸含量、心肌胶原浓度、血管周围胶原面积、心肌胶原容积分数、ρ(Ang)及AT1R蛋白表达水平均显著升高（P0.01）；⑶SO2污染+运动组（SEG）大鼠左室末期舒张压显著升高（P0.01），主动脉收缩压、左室内压峰值、左室内压最大上升/下降速率显著降低（P0.01），心肌局部羟脯氨酸含量、心肌胶原浓度、血管周围胶原面积、心肌胶原容积分数、ρ(Ang)及AT1R蛋白表达水平均显著升高（P0.01），且较SRG大鼠升高更显著（P0.01）。，SO2污染导致运动大鼠心肌胶原纤维形态结构发生异常重塑，最终使大鼠的心功能产生显著的负性变力性效应其机制可能与心脏局部RAS系统的激活有关。 关键词：SO2污染；运动；大鼠；心功能；心肌胶原纤维 中图分类号：G804.34 Effects of SO2 pollution on cardiac function in exercised rats: A Study based on AT1R-mediated remodeling of myocardial collagen fiber morphological structure CHEN Ping1, HU Yanru1,LIU Xiaoli1* 1 College of Physical Education andSports of Bei Jing Normal University, Beijing, 100875, China Abstract:To observe the effects of SO2 pollution on rats’ heart function, from the point of view of the core members of angiotensin Ⅱof cardiac local renin-angiotensin system(RAS) mediated morphological remodeling of myocardial collagen fibers, cardiac function was observed by cardiac intubation technology, the levels of AngⅡ,AT1R protein expression, hydroxyproline content and collagen volume fraction were measured by radioimmunoassay (ELISA),immunohistochemistry and gastric acid hydrolysis. The results showed that：(1)Appropriate exercise to adapt to the myocardial collagen fibers in the morphological structure of adaptive remodeling, and to enhance myocardial contractility, showed that compared with RG, the LVSP, SBP, ± dp/dt max of the rats in EG increased significantly (P0.01),the expression of DBP and AT1R protein levels were significantly decreased (P0.01),the content of HYP, CC, PVCA, CVF, Ang II increased, but the diff