微生物常规鉴定技术（Microbiological routine identification technique）.docVIP

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微生物常规鉴定技术（Microbiological routine identification technique）.doc

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微生物常规鉴定技术（Microbiological routine identification technique）

微生物常规鉴定技术（Microbiological routine identification technique） Morphology, structure and culture characteristics were observed The observation of the morphology of 1, mainly through microbial staining under the microscope on the shape, size, arrangement, cell structure (including cell wall, cell membrane, nucleus and flagellum, such as Bacillus) and staining characteristics were observed, intuitive understanding of bacteria in the structure characteristic, according to different microbes in morphology the different microbial identification difference, to achieve. 2. The cell colony formed on the solid medium surface of bacterial cells is called colony (colony). The growth characteristics of colony formed by different microorganisms in a certain medium are very different, while those of the same species have certain stability under certain conditions. In this way, different microorganisms can be distinguished and identified. Therefore, the observation of microbial culture characteristics is also an important part of microbial identification. 1) bacterial culture characteristics include the following: in the solid culture medium, to observe the colony size and shape and color (pigment is water-soluble or fat soluble), gloss, transparency, texture, shape, edge features and uplift migration etc.. The surface growth in liquid medium (membrane, ring) turbidity and precipitation. The inoculation and diffusion of semisolid medium were observed. (Figure 3-8) Figure 3-8 bacterial culture characteristics 1. point 2. round 3. 4. filamentous irregular 5. rhizoidal 6. spindle 7. flat 8. 9. 10. cushion 11. uplift raised 12. neat edge 13. umbilicated wavy 14. lobed 15. premorse 16. filamentous 17. curly hair 18. silk 19. setiform 20. Beaded 21. Shu chin shaped 22. root shape 23. rhizoids 24. - 25. 26. filamentous Beaded 27. papillary villous 28. roots like 29. cups shaped like 30. radish funnel 32. 33. 31. 34. 35. layered cystic flocculent ring of 36. webbed 37. film 2) the culture ch