hplc过柱的经验（Experience with HPLC over columns）.docVIP

hplc过柱的经验（Experience with HPLC over columns） The column wise remark of an experienced person The column wise remark of an experienced person Since the beginning of 96 years, almost eight years of resistance to the column. Commonly used columns should be called column chromatography, also known as column chromatography. We usually use silica gel or alumina as stationary phase adsorption column. Because of the experience of the column component is too much, so I next years on column experience, write some experience, hope to be helpful. Columns can be divided into: compression, atmospheric pressure, and decompression. Pressure can increase the flow rate of the eluent, reduce the time it takes to collect the product, but reduce the number of trays. So when other conditions are the same, the column is the most efficient, but it has the longest time, such as the separation of natural compounds and a column for a few months. The decompression column can reduce the use amount of silica gel, the feeling can save half or more, but due to the large amount of air through the silica gel will make solvent (sometimes in the post outside, and the condensation of water vapor) some quite easy to decompose the thing may not come, but also to use the water pump to pump gas (a lot of noise, and long time). There used to be a lot of pressure reducing columns, but I had a deeper feeling about it. But since I tried the pressure, I almost never had the idea of reducing pressure again. Compression column is a better method, similar to the atmospheric column, but only additional pressure to make it faster. The supply of pressure can be compressed air, double ball, or a small pump (just air the fish tank). Particularly applicable in the separation of easily decomposed samples. The pressure should not be too large, otherwise, the solvent will go too fast to reduce the separation effect. The individual feels that the compression column is more suitable for the separation of common organic compo