应用合成肽p176对肺包虫病血清学诊断效果分析.PDFVIP

·372 · 中华临床医师杂志( 电子版)2015 年2 月第9 卷第3 期 Chin J Clinicians(Electronic Edition),February 1,2015,Vol.9,No.3 ·临床论著· 应用合成肽p 176 对肺包虫病血清学 诊断效果分析 1 2 2 2 2 赵丹 张生彬 赵金 宋士鹏 周永升 【摘要】 目的 评估合成多肽p176 对肺包虫病的诊断效果。方法 采集了62 份手术确诊的 肺包虫病患者的血清样本，同时采集了 129 份健康志愿者的血清。运用酶联免疫吸附法 （ELISA ） 检测其阳性率。结果 合成多肽p176 诊断肺包虫病的敏感性和特异性分别为79.03%和96.12% 。通 过双变量分析，血清抗体阳性反应和其并发症及囊泡数量有关。通过多因素 Logistic 回归分析，只 有并发症和血清抗体阳性率具有相关性，优势比（OR ）为9.58，95%可信区间（CI）为2.15～42.59 ； P=0.003 。结论 利用合成多肽p176 采用ELISA 方法对诊断肺包虫病的效果良好，值得推广。 【关键词】 棘球蚴病； 酶联免疫吸附测定； p176 1 Serological diagnosis of lung cystic hydatid disease using the synthetic p176 peptide Zhao Dan , 2 2 2 2 1 Zhang Shengbin , Zhao Jin , Song Shipeng , Zhou Yongsheng . Department of Respiratory Medicine, Baotou No. 4 Hospital, Baotou 014030, China; 2Department of Genery Surgery, the Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010, China Corresponding author: Zhou Yongsheng, Email: zyszd_790525@sohu. com 【Abstract 】 Objective The synthetic peptide p176, has shown promising performances for diagnosis of cystic hydatid disease (CHD). We evaluated the performance of the synthetic peptide p176 for the diagnosis of pulmonary hydatid disease in an enzyme-linked immunosorbent assay (ELISA) format. Methods Sixty-two serum samples from patients with a diagnosis of pulmonary hydatidosis confirmed by surgery and 129 from healthy volunteers were tested. Results The overall sensitivity and specificity of the p176 ELISA for lung CHD were 79.03% and 96.12%, respectively. On bivari