裸小鼠脑胶质瘤原位移植瘤模型的建立及磁共振-第三军医大学学报.docVIP

裸小鼠脑胶质瘤原位移植瘤模型的建立及磁共振成像研究 王舒楠，张伟国，陈金华，马长锁，赵丽 (第三军医大学大坪医院野战外科研究所放射科，重庆 400042) 摘要：目的 建立裸鼠脑胶质瘤原位移植瘤模型并进行MR扫描，摸索扫描的最佳参数，探讨1.5T磁共振在检测裸鼠颅内成瘤的可行性，为下一步实验奠定基础。方法 SHG-44细胞传代培养后，用微量注射器吸取肿瘤细胞悬液直接穿刺接种，在肿瘤细胞接种后第15天行1.5T MR扫描，裸鼠麻醉后置于小动物线圈采集图像并利用影像工作站测量体积。而后取脑组织做病理切片，计算体积后行HE及CD34染色，与MRI图像进行对比分析。结果 除去围手术期死亡2只裸鼠外，剩余的13只裸鼠在MR增强序列图像上均能见到肿瘤，成瘤率达100%，且位置同组织切片相一致。在MR图像上测得的肿瘤体积为（29.41±10.95）mm3，组织切片测得的肿瘤体积为(26.57±9.70) mm3，二者无显著性差异（P＞0.05）但具有明显的相关性（r=0.985，P ？）。肿瘤微血管丰富，且主要集中在肿瘤的边缘。结论 采用微量注射器直接建立裸鼠胶质瘤原位移植瘤模型简单便捷，成瘤率高。1.5T磁共振能活体检测裸鼠脑胶质瘤成瘤情况，并能进行一定的功能成像，是动态监测裸鼠胶质瘤发生发展的有效途径。 关键词：裸鼠；胶质瘤；磁共振成像；原位移植 Estabilshment of human glioma orthotopic model in nude mice and monitored by 1.5T magnetic resonance imaging Wang Shu-nan, Zhang Wei-guo,?Chen Jin-hua, Ma Chang-suo, Zhao Li（Department of Radiology, Daping Hospital, The Third Military Medical University, Chongqing 400042, China） Abstract：Objective To establish the model of human glioma orthotopic implantation in nude mice， and investigate 1.5T magnetic resonance in the detection of transplanted tumor. Methods The glioma cell line SHG-44 was cultured in vivo. The glioma cells were injected into nude mice brain using micro-syringe to form glioma. Fifteen days later, nude mice were scaned by 1.5T MR with animal-coil to detect the tumor. Tumor volume was measured in AW4.3 imaging workstation. Then take brain tissue biopsy done with HE staining and CD34 staining. Tumor volnme was measure under microsope to evaluated the relativity with the dates in MR imagings. Results Two nude mice were dead in perioperative, the other thirteen mice survive and be detected tumor in bran by MR scanning with contrast injected. The location in MRI is consistent with that in tissue section. Tumor volume is 29.41±10.95mm3 measured by MRI and is 26.57±9.70mm3 measured under microsope. There is no significant difference（P＞0.05）but significant correlation（r =0.985）. Conclusion It is easy and convenient using micro-syringe directly to establish the human glioma ort