纳米级二氧化钛对雌性体外生殖毒性地研究.pdfVIP

纳米二氧化钛对雌性体外生殖毒性的研究 themshowed results． negative We in-vitrorat follicleculture to theeffectsofTi02 apply preantral systemstudy ontherat and follicle maturation．Underthefoundationof nanoparticle developmentoocyte thiswe theeffectof onthe of cellandifitcan Ti02 study nanoparticleproliferationgranulosa causethechromosomalaberrationof aimofthis wasto whether oocyte．111e study study couldcausefemale evidencesof onits nanoparticle reproductivetoxicity，andprovide Ti02 evaluation． safety 1．Theeffectsoftitaniumdioxide onthe nanoparticle andmaturationofin-vitrorat follicle preantral in-vitrorat follicleculture to theeffectsof OBJECTIVE：Applyingpreantral systemstudy Ti02 ontheratfollicle and maturation． nanoparticles developmentoocyte dissectedovariesto the folliclesand METHODS：Mechanicallygainpreantral allocatedthe folliclesto96一well were of preantral plates．Fivegroups setuprandomly：threegroups and used 25nmTi02(12．5p．g／ml、25p,g／mI5099／m1)；onenegativegroupmedium，another controlusedconventional cultured for10 group Ti02．After subsequentlyindividuallydays， thefollicleswereinduced theconditionoffollicle and ovulation，observed development maturation． oocyte