不同温度处理对温敏核不育水稻广占63S育性表达及其蛋白质组影响.pdfVIP

徐书琴；不同温度处理对温敏核不育水稻广占63S育性表达及其蛋白质组的影响 3 Abstract rice The isaffected and fertility bytemperaturelightduringreproductivestage．At knowlittleaboutthemechanismsof present，we temperature—and／or light—regulated conversion．In the of ofrice fertility study,we present Investigatedchangesproteome transitionof athermo—sensitivemalesterilerice spikeletduring fertilityusing genie triedto that variety,Guangzhan63s，and regulated explorepossibleproteins fertility transitionoffice．themain as resultsarefollows： 1．As rice treated砸th than compared谢thplants llightemperature(more26C) thermo—sensitive Increased during stage．Lowtemperature as the and as Wasobserved． fertilityhigh 25．7％feritily 2．Inordertoisolate whole of rice efficientlyproteins spikelets，wefirstly establishedand the oftwo—dimensional optimizedtechniqueplatform rice first the dimentional (2一DE)forspikelet is，for proteomicanalysis，that immobilizedlinear Was4-7andthe of electrophoresis，thepre-cast pHgradient length