壳聚糖金属配合物对黑曲霉的抑制活性研究-食品科学.pdfVIP

152 2011, Vol. 32, No. 03 食品科学 ※生物工程 1 1 1 , 2 3 1 冯小强 ，李小芳 ，杨 声 ，伏国庆 ，王廷璞 (1.天水师范学院生命科学与化学学院，甘肃 天水 741001 ；2.定西师范高等专科学校化学系， 甘肃 定西 743000 ；3.天水市中医医院，甘肃 天水 741001) 通过观察黑曲霉菌丝体的生长及孢子萌发情况，考察壳聚糖(CS)及壳聚糖锌(CS-Zn) 、壳聚糖镍(CS-Ni) 的 抗真菌性能。结果表明：壳聚糖、壳聚糖锌和壳聚糖镍对黑曲霉孢子萌发和菌丝体生长均具有明显抑制作用，且 壳聚糖金属配合物的抑菌效果比壳聚糖本身更强，其中壳聚糖镍最强。壳聚糖镍对黑曲霉的抑菌活性受壳聚糖分子 质量、环境 pH 值、质量浓度因素的影响较大。分子质量为 5kD 的CS 制备的 CS-Ni 抗真菌效果最好；CS-Ni 抑菌 效果在pH3 ～7.5 时随pH 值降低而增强；随 CS-Ni 质量浓度(0～0.75mg/mL) 的增加而增强。 壳聚糖金属配合物；黑曲霉；抗菌 Anti-Aspergillus niger Activity of Metal/Chitosan Complexes 1 1 1,2 3 1 FENG Xiao-qiang ，LI Xiao-fang ，YANG Sheng ，FU Guo-qing ，WANG Ting-pu (1. College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China ；2. Department of Chemistry, Dingxi Teachers College, Dingxi 743000, China；3. Traditional Medical Hospital of Tianshui, Tianshui 741001, China) Abstract ：Chitosan and metal/chitosan complex were evaluated comparatively for their anti-Aspergillus niger activity based on the mycelial growth and spore germination of the fungus. The results showed that both chitosan and metal-chitosan could effectively inhibit the mycelial growth ofA. niger and its spore germination, while chitosan displayed lower antifungal activity than its complexes with Zn or Ni, and chitosan/ Ni complex showed the strongest antifungal activity. This biological activity of chitosan was largely influence by its molecular weight and concentration as well as environmental pH. The complex between chitosan with 5 kD molecular weight and Ni had the best inhibitory effect on A. niger . The i