重组马白细胞介素-18生物学活性的测定和单克隆抗体中和活性鉴定.pdfVIP

农业生物技术学报JournalofAgriculturalBiotechnology2009，17(3)：370-374 ·研究论文· 重组马白细胞介素．18生物学活性的测定及单克隆抗体中和活性鉴定 童铁钢，白字，王群，张维军，刘光亮．徐树兰，林燕，孙庆歌，杨涛．吴东来¨ (中国农业科学院哈尔滨兽医研究所，农业部兽医公共卫生重点开放实验室，兽医生物技术国家重点实验室。哈尔滨150001) 生物学活性，并且呈一定的剂量依赖性。 关键词：马白细胞介素．18；生物学活性；单克隆抗体 中图分类号：S188，S185文献标识码：A文章编号：1006．1304(2009)03．0370．05 DeterminationofRecombinantInterleukin一18 BiologicalActivity Equine and Antibodies IdentificationofNeutralizedofMonoclonal Activity TONG Tie—gang，BAIYu，WANGQun，ZHANGWei-jun，LIUGuang—liang， XU Shu-lan，LINYan，SUN Tao，WU Qing-ge，YANGDong-lai宰· (The Public KeyLaboratoryofVeterinaryHealth，MinistryofAgriculture,StateKeyLaboratoryofVeterinaryBiotechnology, Harbin Research Science,Harbin150001，China) VeterinaryInstitute,ChineseAcademyofAgricultural AbCxa比Toexaminethe ofrecombinant fromEscherichia was bioactivity equineinterleukin-18(rEIL广18)obtainedcoli,rEIL-18 evaluatedtoseeifitshoweda effectwithrecombinanthuman theinduction inteffer- synergistic interleukin—12(rhlL-12)forofequine from bloodmononuclear Real-timeRT—PCR．REIL-18couldin- On一丫(EIFN-7)geneexpressionequineperipheral cells(PBMCs)by duce from PBMCsabout20一fold thanthatof controlinthe ofrhIL-12． EIFN·1，mRNAexpressionequine higher negative presence And toinduce was withrEILl8inafixedconcentrationofrhlL-12．Nineall- theeffect EIFN一1，geneexpressiondose—dependent ti-rEIL-18monoelonal alsoevaluatedcouldneutralizethe ofrEIL-18．An