响应面分析法优化光皮木瓜总酚醇提工艺的研究-中国酿造.PDFVIP

2009 No．2 · · Serial No．203 China Brewing Research Report 76 响应面分析法优化光皮木瓜总酚醇提工艺的研究 刘丽丽，张建新*，王 娜，郑海燕 （西北农林科技大学食品科学与工程学院，陕西 杨凌 712100） 摘 要：依据响应面试验设计法，选取浸提温度（x ）、固液比（x ）、乙醇浓度（x ）、浸提时间（x ）为考察因素，进行光皮木瓜总酚醇提工 1 2 3 4 艺优化试验。在分析各个因素的显著性和交互作用后，得出光皮木瓜总酚醇提的最佳工艺条件为：浸提温度69.28℃ ，固液比31.6 （g:mL），乙醇浓度51.34%vol，浸提时间1.58h；各因素的主效应关系（Y）为：浸提温度＞固液比＞乙醇浓度＞浸提时间。建立的总酚醇 提数学模型为：Y = 122.70 + 5.61x + 0.50x - 2.11x 2 - 1.39x 2 - 2.65x 2 - 2.71x 2 - 0.68x x - 0.83x x + 1.02x x ，总酚得率的预测值为 1 2 1 2 3 4 1 2 1 4 2 3 125.73mg/g ，验证值为127.53mg/g 。理论值与验证值的复相关系数为0.9948 ，证明此模型是合理可靠的，可用于实际预测。 关 键 词：光皮木瓜；总酚；醇提；响应面法；优化 中图分类号：O625.31 文献标识码：A 文章编号：0254－5071 （2009）02-0076-04 Optimization of total phenol alcohol extraction from chaenomeles sinsis with response surface methodology LIU Lili, ZHANG Jianxin*, WANG Na, ZHENG Haiyan (College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling 7 12 100, China) Abstract: The process of total phenol alcohol extraction from chaenomeles sinesis was studied using the response surface method, in which tempera- ture （x ）, solid-liquid ratio （x ）, alcohol concentration （x ）and extraction time （x ）were selected as experimental factors. Based on significance and 1 2 3 4 interaction of each factor, the optimum conditions of alcohol extraction were obtained as followed: extraction temperature 69.28℃, solid-liquid ratio 31.6 （g:mL）, alcohol concentration 51.34% （vol）and extraction time 1.58h