绿茶多酚微波辅助提取和纯化工艺研究.pdfVIP

中国农学通报 2010,26(19):66-69 Chinese Agricultural Science Bulletin 绿茶多酚微波辅助提取和纯化工艺研究 王海燕，刘焕云，韩 帅 （河北经贸大学生物科学与工程学院，石家庄050061） 摘 要：以碎绿茶为原料，采用二步法提取和纯化茶多酚，通过正交试验确定较佳工艺为：第一步采用微 波乙醇溶液提取效果最好，最佳条件是乙醇溶液浓度为50% ，料液比为1:30 ，浸提20 min 后，用320 W 微 波辐射25 s ，再常温浸提60 min ，重复浸提2 次，在此工艺条件下茶多酚浸出率为21.74% 。第二步采用 3+ 2+ 离子沉淀法纯化茶多酚，最佳工艺条件为选用A 1 +Zn 复合沉淀剂，沉淀剂加入量与茶叶质量之比为 3:20 ，沉淀温度在25℃左右，当茶多酚沉淀完全后迅速分离，最终得到的茶多酚浓缩液纯度可达 99.50%。 关键词：茶多酚；提取；微波；离子沉淀 中图分类号：TS202.3 文献标志码：A 论文编号：2010-1087 Microwave-assisted Extraction and Purification Research of Tea Polyphenols Wang Haiyan, Liu Huanyun, Han Shuai (College of Biology science and Engineering, Hebei Economics ＆trade University, Shijiazhuang 050061) Abstract: To extract and purify the tea polyphenols from green tea, two-step extraction technology was studied. The optimum extraction process was determined using the single factor and orthogonal experiments. The results show that the first step for microwave-ethanol extraction is optimum, the best conditions for ethanol concentration 50%, solid-liquid ratio l:30, extraction 20 min later, with 320 W microwave radiation 25 s, and then extraction at room temperature 60 min, repeat the extraction two times, under this optimum conditions, the extraction rate of TP is 21.74% . For the second step, TP is purified by ion precipitation, the optimum 3 + 2 + conditions for the selection of A1 +Zn , the mass ratio of precipitant to tea is 3:20, precipitation temperature around 25℃, TP is separated as quickly as possible when it is precipitated c