人子宫颈癌pd-l1的表达及其与肿瘤内.docVIP

人子宫颈癌PD-L1的表达及其与肿瘤内浸润T细胞的相关性研究 [摘要] 目的 共刺激分子B7家族成员之一 程序性死亡受体1分子（programmed death receptor-ligand 1，PD-L1）通过PD-L1/PD-1途径负性调节T细胞的免疫功能。本文拟观察子宫颈瘤变上皮和子宫颈癌细胞PD-L1表达、病灶内浸润淋巴细胞PD-1的表达，PD-L1与局部CD4+T和CD8+T细胞浸润的关系，以及PD-L1可能对肿瘤浸润淋巴细胞凋亡的影响。方法 免疫组织化学染色观察5例正常宫颈组织、7例高级别CIN(CINⅡ-Ⅲ)组织和67例子宫颈癌组织中PD-L1的表达和淋巴细胞PD-1的表达，免疫荧光染色观察相应组织内浸润的CD4+T和CD8+T细胞数量，TUNEL法检测淋巴细胞凋亡。结果 正常子宫颈上皮不表达PD-L1，宫颈瘤变上皮不表达或弱阳性表达PD-L1，70%（47/67）的宫颈癌表达PD-L1。部分肿瘤浸润性淋巴细胞表达PD-1。子宫颈瘤变上皮与子宫颈鳞状细胞癌PD-L1表达存在明显差异（P＜0.01），浅表浸润的子宫颈鳞状细胞癌PD-L1的表达略低于深部浸润的鳞状细胞癌，但无显著差异；PD-L1阳性病例肿瘤局部CD8+T细胞数量显著减少，且PD-L1表达与CD8+T细胞数量明显负相关（r=-0.82，P0.01）r =-0.05，P0.05）但与CD4+T细胞数量无关。PD-L1表达可能与肿瘤内浸润的淋巴细胞凋亡有关。 [关键词]子宫颈癌；PD-L1；CD4+T cell；CD8+T cell；凋亡 [中图法分类号] [文献标志码]A An investigation of PD-L1 expression and its relationship with tumor infiltrating T cells in human cervical carcinoma Wang Yuqi，Xu Guangu，Xu Man (Department of Pathology， Molecular Medicine and Cancer Research Center， Chongqing Medical University， Chongqing 400016) Abstract] Purpose Programmed death receptor-ligand 1(PD-L1)， one member of co-stimulatory molecules B7 family， negatively regulates T cells’ immune function via PD-L1/PD-1 pathway. This study intends to observe the expression of PD-L1 and PD-1， and the relationship between PD-L1 and CD4+T or CD8+T cell infiltration in the lesion of CIN and cervical carcinoma， as well as the impact of PD-L1 on the apoptosis of tumor infiltration lymphocytes. MethodsThe expression of PD-L1 and PD-1 were observed respectively in five cases of normal cervical tissue， 7 cases of high-level CIN (CIN Ⅱ-Ⅲ) and 67 cases of cervical carcinoma by immunohistochemistry staining; the infiltrating of CD4+T and CD8+T cell were determined by immunofluorescent staining， and the apoptosis of tumor infiltration lymphocytes were examined by TUNEL assay in those cases. ResultsThere was no expression of PD-L1 in normal cervical epithelium， negative or weak positive expression of PD-L1 in epithelia of those high grade CIN cases， and