肝富集转录因子对hbv基因转录和复制的调控作用.pdfVIP

　2009 12 18 12 　ChinJGastroenterolHepato,lDec2009, Vo.l18, No.12 ·1079 · HBV 1 2 3 3 王　甦 , 陈　群, 唐　红,赵连三 1.扬州大学医学院附属苏北人民医院消化科, 江苏 扬州 22500 1;2.扬州大学医学院基础医学部;3.四川大学华 医院感染科 【】　　(HBV), HBV。 　 HBV pHBV4.1 HNF3、HNF4 RXRα/PPARα NIH3T3。 Northern HBV3.5 k、2.4/2.1 k、0.7 kmRNA, Southern HBVDNA 。　 HBV pHBV4.1 3.5 kHBVRNA HBVDNA; pHBV4.1 NIH3T3, 3.5 kHBVRNA, HBVDNA ;, HNF4 RXRα/PPARα 3.5 kHBVRNA HBVDNA, HNF3 HBV, HNF4 RXRα/PPARα, 3.5 kHBVmRNA HBVDNA 。　 HBV。, HNF4 RXRα/PPARα HBV;HNF3 HBV。 【】　;;; :R512.6+2　　　:A　　　:100 6-5709 (2009 )12-1079-04　　　:2009-04 -2 3 Theregulatoryroleofliver-enrichedtranscriptionfactorsinHBVgenetranscriptionandreplica- tion 1 2 3 3 WANGSu, CHENQun, TANGHong, ZHAOLiansan 1.DepartmentofGastroenterology, SueiPeople sHospitalAffiliatedtoMedicalColleg,eYangzhouUniversit,yYang- zhou225001;2.DepartmentofBaseMedicineofMedicalColleg,eYangzhouUniversity;3.DepartmentofInfectious Diseas,eWestChinaHospita,lSichuanUniversity, China 【A stract】　O jective　ToestalishhepatitisBvirus(HBV)replicationsystemwithnonhepaticcelllinesandto studytheregulatoryroleofvariousliver-enrichedtranscriptionfactorsinHBVgenetranscriptionandreplication.Meth- ods　Thereplication-competentHBVrecom inantplasmidpHBV4.1 plusdifferentliver-enrichedtranscriptionfactor (HNF3, HNF4 andRXRα/PPARα)expressionplasmidswereco-transfectedintononhepaticcelllinesrespectively (NIH3 T3 ).Thetranscriptionlevelsof3.5 k, 2.4 /2.1 k and0.7 kHBVmRNAwereanalyzedyNorthern lot hyridization, andthelevelsofHBVDNAreplicationintermediatesweredetected ySouthern lothyridization.Re- sults　3.5 kHBVRNAreplicationproductsandHBVDNAintermediatescouldedetectedinhepaticcelltransfected yreplication-competentHBVrecom inantplasmidpHBV4.1.Howeve,rintheasenceofco-