真正柑橘果树类植物基于AFLP分子标记的分类与进化研究.PDFVIP

植物分类学报 46 (5): 682–691 (2008) doi: 10.3724/SP.J.1002.2008.07135 Journal of Systematics and Evolution (formerly Acta Phytotaxonomica Sinica) 真正柑橘果树类植物基于AFLP 分子标记的分类与进化研究 1,2 1,2 * 1 谢让金 周志钦 邓 烈 1( 中国农业科学院-西南大学柑橘研究所 重庆 400712) 2(西南大学园艺园林学院 重庆 400716) Taxonomic and phylogenetic relationships among the genera of the True Citrus Fruit Trees Group (Aurantioideae, Rutaceae) based on AFLP markers 1,2Rang-Jin XIE 1,2Zhi-Qin ZHOU * 1Lie DENG 1(Citrus Research Institute, Chinese Academy of Agricultural Sciences, Southwest University, Chongqing 400712, China) 2(College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China) Abstract Fifty-nine genotypes representing six genera of the True Citrus Fruit Trees Group (Aurantioideae , Rutaceae) were analyzed using AFLP technique to study their taxonomic and phylogenetic relationships. Fifteen primer combinations (out of 64 screened) were selected based on the polymorphism and quality of the bands produced by the primer and used in the present study. A total of 312 bands were obtained, of which 305 (97.8%) were polymorphic. The UPGMA tree of all genotypes was constructed based on the AFLP data using PAUP* beta version 4.0b8 software. Our AFLP molecular tree clearly confirms that the True Citrus Fruit Trees Group is monophyletic and supports the division of the group into genera mainly based on morphological characters, except that the genus Fortunella was nested wit