新型血小板保存液对高浓度血小板低温液态-第三军医大学学报.docVIP

新型血小板添加液对血小板低温液态保存效果的实验研究 王昕 李静 史蓉华 赵树铭* （第三军医大学附属西南医院输血科，重庆 400038） 【摘要】目的 探讨使用改良的以海藻糖为添加剂的血小板添加液替代自体血浆在低温条件下的保存效果。方法 采集6单位单采血小板，每一单位单采血小板平均分为3份，一份加入70%PAS-ⅢM/30%血浆10℃保存一份加入血浆常温（22℃）保存另一份加入血浆和海藻糖－85℃保存；组在第1天、5天、7天和9天取样检测，组在20天后复苏取样检测。分别检测PLT、PDW、MPV、CD62p、HSR、PAgT、pH值、LDH以及GLU的变化。结果 保存期内随保存时间延长，组和组CD62p表达增加，HSR和最大聚集率降低；组的LDH释放、GLU消耗、CD62p表达、血小板最大聚集率与组有显著差异（P0.05）。组pH在第5天起明显降低（P0.05）。组LDH释放明显较其他两组增多，但CD62p表达较少（P0.05）。其他检测指标PLT, MPV, PDW相差不显著。结论 改良的PAS-ⅢM能够很好的替代血浆用于血小板的保存，同时在低温条件下能够很好保护血小板的功能，保存效果好于血浆常温保存。 【关键词】血小板；PAS；低温保存；海藻糖 Experimental research on the effect of modified PLT additive solutions store platelets concentrates at low temperature WANG Xin, LI Jing, SHI Rong-hua, ZHAO Shu-ming （Department of transfusion, Southwest hospital, Third Military Medical University, ChongQing, China） 【Abstract】 Objective To use modified PLT additive solutions(PAS-ⅢM) by adding trehalose as a substitute for plasma for the storage of platelet concentrates . Methods Apheresis platelets from 6 donors were divided and stored in different media(A:70%PAS-ⅢM/30%plasma , B:plasma, C:plasma/trehalose), and each media group stored at 22℃, 10℃ and －85℃ respectively. The samples of each platelets were respectively collected on day,0 day 1, day 5, day 7, day 9 after storage (samples of platelets stored at －85℃ were collected on day 20 after storage) and platelet count, mean platelet volume, platelet distributing width, CD62p, hypotonic shock response, platelet aggregation, pH, lactic dehydrogenase, glucose were evaluated. Results The expression of CD62p of A group and B group were increased in a time-dependent manner, but HSR and platelet aggregation were decreased. The expression of CD62p, LDH release, GLU consumption of B group were significant higher than that of A group(P0.05). HSR, platelet aggregation of B group were significant lower than that of A group(P0.05). After 5 days, pH of B group were significant decrease(P0.05). LDH release were significant high in samples of C group an