反向遗传技术在猪繁殖与呼吸综合征研究中的应用-中国农学通报.pdfVIP

中国农学通报 2011,27(7):364-368 Chinese Agricultural Science Bulletin 反向遗传技术在猪繁殖与呼吸综合征研究中的应用 谷元兴，王 猛，刘永生，张 杰 （中国农业科学院兰州兽医研究所，家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室， 农业部兽医公共卫生重点开放实验室，兰州730046） 摘 要：猪繁殖与呼吸综合征（PRRS ）由猪繁殖与呼吸综合征病毒（PRRSV ）引起，严重危害全世界养猪 业。PRRSV 与宿主细胞的相互作用、致病机理，其基因组及蛋白质对致病性的影响等仍是未解之谜。 反向遗传技术为研究PRRSV 提供了技术支撑。现基于病毒拯救、疫苗研制、分子病原学诊断等方面的 内容，对反向遗传技术在PRRS 研究中的应用作一综述。 关键词：反向遗传技术；病毒拯救；RNA 病毒；猪繁殖与呼吸综合征；应用 中图分类号：S852.65+9.6 文献标志码：A 论文编号：2010-2592 ApplicationofReverseGeneticsManipulationintheResearchofPorcineReproductive andRespiratorySyndrome Gu Yuanxing, Wang Meng, Liu Yongsheng, Zhang Jie (KeylaboratoryofVeterinaryPublicHealthofAgriculture,KeyLaboratoryofAnimalVirologyofMinistryofAgriculture, StateLaboratoryofVeterinaryEtiologicalBiology,LanzhouVeterinaryResearchInstitute, ChineseAcademyofAgriculturalSciences, Lanzhou 730046) Abstract: Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is considered one of the most important contagious diseases in swine industry today and responsible for significant economic losses worldwide. Reverse genetics manipulation has been used extensively in variety of fields of life science for several decades, which supported us a powerful method to explore organisms. This paper mainly summarized the application and perspective of reverse genetics manipulation in the research of PRRS, including virus rescue, vaccines development, molecular etiology diagnosis and so on. Keywords:reverse genetics manipulation; virus rescue; RNA virus; PRRS; applications 0 引言 明两种毒株间存在着显著差异,