血清中时视黄醇结合蛋白免疫透射比浊测定法-上海北加生化试剂有限.docVIP

血清中视黄醇结合蛋白免疫透射比浊测定法 王晓蓉 (上海电力医院 上海 200052) 摘要：目的 对视黄醇结合蛋白免疫透射比浊测定法进行方法学和临床应用评价。方法 采用免疫透射比浊测定法，根据NCCLS相关文件，对方法进行精密度、线性、准确性等评价及临床初步应用。结果 批内CV 1.5%~2.2% ，批间CV 2.8%~3.7% ，日间CV 2.6%~3.1% ，总CV 4.5%~6.7% ，线性范围0至210 mg/L , r =0.9985 ,对照组血清视黄醇结合蛋白含量：49.5±11.9 mg/L；糖尿病肾病组：81.0±12.8 mg/L；慢性肾小球肾炎组：133.8±22.8 mg/L；急性肾小球肾炎组：175.4±45.2 mg/L；慢性肾功能衰竭：88.4±30.7；肾病组与对照组有显著差异（P〈 0.01）。结论 免疫透射比浊法测定血清视黄醇结合蛋白，方法简便、快速、灵敏，结果准确，可用自动分析仪测试，适合临床检验科应用。对肾脏疾病的早期诊断有一定临床意义。 关键词 视黄醇结合蛋白 免疫透射比浊法 肾功能 Turbidimetric Immunoassay for determination of Retinol Conjugated Protein in Serum Xiaorong Wang . ( Shanghai Power Hospital ，Shanghai, 200052，China) Abstract：Objective: To evaluate the methodology and clinical application of turbidimetric immunoassay for determination of retinol conjugated protein in serum. Method: According to NCCLS relative documents, evaluate the precision, linearity and accuracy of this turbidimetric immunoassay and try a clinical application. Results: within-lot CV 1.5%-2.2%, between-lot CV 2.8%-3.7%, between-day CV 2.6%-3.1%, total CV 4.5%-6.7%, linearity range 0-210mg/L, r = 0.99985. Quantity of retinol conjugated protein in Compared Group: 49.5±11.9mg/L; Diabetic Renopathy Group: 81.0±12.8mg/L; Chronic Glomerulonephritis Group: 133.8±22.8mg/L; Acute Glomerulonephritis Group: 175.4±45.2 mg/L； Chronic Renal Failure Group: 88.4±30.7mg/L. There’s obvious difference between the kidney disease Group and the Compared Group. (P 0.01) Conclusion: The method to determine retinol conjugated protein by turbimidetric immunoassay is simple, quick, sensitive, and accurate-resulted. So it can be applied on automated analyzer in clinical testing laboratory. Also it has certain clinical significance for the preliminary diagnosis. Key words: Retinol Conjugated Protein Turbidimetric Immunoassay Renal Function 人视黄醇结合蛋白（Retinol – binding Protein , RBP）分子量约21,000 ，半衰期3~12h，等电点PH4.4~4.8，由肝细胞合成，分布在人的血清、脑脊液、尿液等[1]。在血液中，RBP与视黄醇（VitA）、甲状腺素转运蛋白按1：1：1结合，以复合物形式存在，能携带90%视黄醇，从肝脏向周围组织运送