家鸡全基因组测序研究进展-生物技术通报.pdfVIP

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN ( ) 2017, 33 7 :35-39 家鸡全基因组测序研究进展 李东华  王鑫磊  李转见  孙桂荣  康相涛  闫峰宾 （河南农业大学牧医工程学院 河南省家禽种质资源创新工程研究中心，郑州 450002） 摘 要 ： 鸡具有独特的生物学特性。全基因组测序是即对一种生物的基因组中的全部基因进行测序，主要包括de novo 测序 和全基因组重测序。近年来，由于测序技术的飞速发展，鸡全基因组研究取得了很多重要的进展，为解释鸡的生物学特性和缩短 分子育种周期发挥了重要作用。重点阐述了不同品种鸡全基因组de novo 测序的完成、全基因组重测序技术在解析品种遗传多样性、 揭示进化机制、质量性状遗传机制广泛应用，讨论了鸡全基因组测序工作存在的问题及其展望，旨在为家鸡种质资源的改良和分 子育种提供重要的参考资料。 关键词 ： 鸡 ；基因组 ；测序 DOI ：10.13560/ki.biotech.bull.1985.2017-0194 Research Advances on Whole Genome Sequencing of Chicken LI Dong-hua WANG Xin-lei LI Zhuan-jian SUN Gui-rong KANG Xiang-tao YAN Feng-bin （College of Animal Science and Veterinary Medicine ，Henan Agricultural University ，Henan Innovative Engineering Research Center of Poultry Germplasm Resource ，Zhengzhou 450002 ） Abstract: Chicken possesses unique biological traits. Whole genome sequencing is the sequencing of all genes in a biological genome， mainly including de novo sequencing and whole genome re-sequencing. With the rapid development of sequencing technology in recent years，there have been great progresses in chicken genome research，which plays a critical role in explaining the biological characteristics of chicken and shortening the period of molecular breeding. This paper mainly describes the de novo sequencing completion of whole genome in different chicken cultivars，as well as the wide application of whole genome re-sequencing technology in analyzing genetic diversity，revealing evolutionary mechanisms and genetic mechanisms of quality traits，and further discusses the existing issues and potential prospects in the chicken whole genome sequencing. The aim is to provide a resource for genetic im