苹果两个新s-rnase基因克隆与46个品种s基因型的pcr-中科瑞泰.pdfVIP

农业生物技术学报，2010 年，第18 卷，第2 期，第265-271 页 Journal of Agricultural Biotechnology, 2010, Vol.18, No.2, 265-271 研究报告 A Letter 苹果两个新 -RNase 基因克隆与46 个品种S 基因型的PCR 分析 S 1 1 1 2 3 1* 龙慎山 李茂福 韩振海 张冰冰 王昆 李天忠 1 中国农业大学果树细胞与分子育种实验室, 北京, 100193; 2 吉林省农业科学院果树研究所, 公主岭, 136100; 3 中国农业科学院果树研究所, 兴城, 125100 * 通讯作者, litianzhong1535@163.com 摘 要 蔷薇科苹果是一个典型的配子体型自交不亲和性果树树种，确定苹果品种的S 基因型对于合理 配置授粉树和杂交育种亲和性亲本选择具有重要意义。研究从苹果(Malus domestica) 品种达尔文和金沙依 拉姆中克隆得到了两个新 -RNase 基因，分别命名为 -RNase 和 -RNase ，它们在GenBank 上的登录号分 S S53 S54 别为FJ602074 和FJ602075 ，并对46 个苹果品种基因组DNA 进行 等位基因特异性PCR 扩增，鉴定了其S S 基因型。 关键词 苹果, 自交不亲和性, S-RNase 基因克隆, S-基因型鉴定 Characterization of Two Novel S-RNase Genes and PCR Analyzing of S-genotypes of 46 Cultivars in Malus domestica Borkh. Long Shenshan 1 Li Maofu 1 Han Zhenhai 1 Zhang Bingbing 2 Wang Kun 3 Li Tianzhong 1* 1 Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193; 2 Institute of Pomology of the Jilin Academy of A- gricultural Sciences, Gongzhuling, 136100; 3 Fruit Tree Research Institute of the Chinese Academy of Agricultural Sciences, Xingcheng, 125100 * Corresponding author, litianzhong1535@163.com DOI: 10.3969/j.issn.1674-7968.2010.02.010 Abstract Apple (Malus domestica Borkh) is a typical Rosaceae species that exhibits gametophytic self-incompat- ibility (GSI). Identifying the S-genotypes of apple cultivars have the practical significance for selecting appropriate pollinated varieties and incompatibility parents for hybridization breeding. In this investigation two novel genes named S53-and S54-RNase respectively were cloned from apple cultivars Darling and Jinshayilamu. They were de- posited in GenBank under the accession numbers of FJ602074 and FJ602075,