褐纹报春苣苔组织培养与快速繁殖-广西植物.docVIP

褐纹报春苣苔组织培养与快速繁殖 闫海霞，邓杰玲1，黄昌艳1，关世凯1，何荆洲1，张自斌1，卜朝阳1＊ （1广西壮族自治区农业科学院花卉研究所，南宁，530007） 摘要：褐纹报春苣苔(Primulina glandaceistriata)是一种极具观赏价值的喀斯特地区野生花卉，目前尚未有褐纹报春苣苔组培快繁的研究以褐纹报春苣苔的叶片为外植体，通过两种途径建立其组培快繁体系。结果表明：适宜的不定芽诱导培养基为MS+6-BA 2.0 mg·L﹣1+NAA 0.10 mg·L﹣1，适宜的不定芽增殖培养基为MS+ZT 2.0 mg·L﹣1+NAA 0.10 mg·L﹣1+活性炭 0.05 g·L﹣1，增殖系数为11.09；适宜的愈伤组织诱导培养基为MS+TDZ 2.0 mg·L﹣1+NAA 0.10 mg·L﹣1，适宜的愈伤组织分化培养基为MS+ZT 1.0 mg·L﹣1+NAA 0.10 mg·L﹣1，分化系数为12.46；适宜的生根培养基为1/2MS+IBA 0.1 mg·L﹣1+活性炭0.05 g·L﹣1或1/2MS+IBA 0.5 mg·L﹣1+活性炭0.05 g·L﹣1，生根率为100 %。研究褐纹报春苣苔为今后褐纹报春苣苔的遗传转化提供技术支持。 关键词：褐纹报春苣苔不定芽愈伤组织 Study on tissue culture and rapid propagation of Primulina glandaceistriata （Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007）Yan Hai-xia1, Deng Jie-ling1, Huang Chang-yan1, Guan Shi-kai1, He Jing-zhou1, Zhang Zi-bin1, Bu Zhao-yang1＊ Abstract: Primulina glandaceistriata is a highly ornamental value of the wild flowers in Karst, yet there is research on the tissue culture of Primulina glandaceistriata. The experiment took the leaves of Primulina glandaceistriata as material to research tissue culture and rapid propagation by two methods. The results showed that the optimum medium for adventitious bud regeneration was MS+6-BA 2.0 mg·L﹣1mg·L﹣1he optimal hormone assorted proportion for multiplication culture was MS+ZT 2.0 mg·L﹣1mg·L﹣1active carbon 0.05 g·L﹣1 and the ultiplication coefficient was 11.09, the optimum culture media which induced the callus was MS+TDZ 2.0 mg·L﹣1mg·L﹣1he best differentiation media was MS+ZT 1.0 mg·L﹣1mg·L﹣1 and the ifferentiation coefficient was 12.46, the optimum medium for rooting culture was 1/2MS+IBA0.1 mg·L﹣1 active carbon 0.05 g·L﹣1mg·L﹣1 active carbon 0.05 g·L﹣1ooting rate was 100%. In this study, tissue culture and rapid propagation system of Primulina glandaceistriata was successfully developed to provide a theoretical basis and technical support for future research on genetic transformation. Key words: Gesneriaceae, Primulina glandaceistriata, tissue cult