池蝶蚌cypa的应激表达及对hela细胞生长的影响-水生生物学报.pdfVIP

39 3 Vol. 39, No.3 2 0 1 5 5 ACTA HYDROBIOLOGICA SINICA May, 2 0 1 5 doi: 10.7541/2015.63 CyPA Hela 1 1 1 1 1 1 罗 春 徐 灵 何 静 史建伍 盛军庆 彭 扣 王军花1 2 1 黄 滨 洪一江 (1. , 330031; 2. , 330046) : (Hyriopsis schlegelii)A(Cyclophilin A, CyPA)Hela , Real-time PCR CyPA mRNA , , CyPA 4h , 8h , HsCyPA ; pET-32a(+), HsCyPA cDNA , (ORF) BL21(DE3) , , ; MTT , CyPA (50100200400 800 ng/mL), Hela , CyPA Hela , 100 ng/mL , 18.5%, CyPA : ; A; Real-time PCR; ; Hela ; MTT 中图分类号: S944.4 文献标识码: A 文章编号: 1000-3207(2015)03-0475-07 (Cyclophiins, Cyps) , , , [9] , CyPA A(Cyclophilins, CsA) , CsA[1] , CyPA [12]; , CyPA[13], [2] CyPA, CyPA CyPA Qian [3] RNA CyPA [10, 11] , , , CypA [14] HsCyPA cDNA [4] [5] , CyPA 1229 bp, 164 [6] [7] [8] ; CyPA, ,