凤尾菇漆酶性质及应用的研究-计算机工程与科学.pdfVIP

福建农业学报23( 1) : 8~ 52, 2008 : 1008- 038 ( 2008) 01- 00 8- 05 Fuj ian Journal of Agricultural Sciences 朱海潇, 黄桂英, 王 霖, 颜 松, 胡开辉 ( , 350002) : Pleurotus eryngii ( ) , , 27 U mL- 1 ABTS pH 5, 65 , 60 pH 5~ 60 , ABTS km 0335 mmolL- 1 , Vmax 0795 molL- 1 min- 1 , ~ 5 min , ABTS , , , 05 mmol L- 1 ( ) , 723% 50% , ABTS : ; ; ; ; : S 6 61 3 : A The studies on characterization and Application of edible fungi- P leurot us ery ng ii ZH U Hai-xiao, HUANG Gui-ying, WANG Lin, YAN Song, HU Kai-hui ( College of Lif e Science, Fuj ianA griculture and Forestry University , Fuzhou, Fuj ian 350002, China) Abstract: In t he research, By fermentation of Pleurotus eryngii ( Fengjie NO 1) , and Futher studies on the charac- terization and decolor ability w ere carried out with this strain With preliminary purification by salting out, dialyzing 1 and condensing, the enzyme activity reached 27 U mL T he enzyme is stable below 60 and in the pH range of 5- 60The optimum pH and t em perat ure for oxidation of ABT S w ere 5 and 65 , respectivelyThe km value - 1 - 1 - 1 is 0335 mmolL and Vmax is 0795 molL min w ith ABTS as the substrate. The react ion time is - 5 min. Under different substrates, ABTS displayed maximum oxygenation capability , Guaiacol, Hydroquinone, cat- echol take second place, Ferulic acid have no oxygenation capabilit y Sodium azide is the most eff icient inhibitor, which can rest rain laccase activity completely at 05 mmol L- 1. T he laccase could decolor t riphenylmethane ( mal- achite green stain, cryst al violet) And from the research, The laccase decoloring malachite green stain is