杨树不同组织中arf表达与生长素相关性研究-广东农业科学.pdfVIP

134 广东农业科学 2013 年第 6 期 杨树不同组织中ARF 表达与生长素相关性研究 李晓燕 陈雪梅 盖 颖 北京林业大学生物科学与技术学院袁北京 100083冤 摘要 木质素的生物合成是一个复杂的过程袁有许多酶参与了此过程遥 生长素响应因子 ARFs冤在木质素合成过程中起着重 要 用袁通过调节早起生长素基因的表达袁从而影响基因的转录活化或抑制遥 利用A RFs 已有序列及杨树基因芯片数据对其进化 及与生长素的相关性进化分析袁结果表明袁杨树A RF 序列的保守性高达 60% 曰生长素含量尧上调基因数目与基因表达信号量两两 之间在夏秋季节中表现为高度相关袁冬季对生长素响应因子信号量的影响最大遥 关键词 生长素响应因子曰 杨树基因芯片曰 生长素曰 相关性 + 中图分类号 Q946.885 .1 文献标识码 A 文章编号 员园园源原愿苑源载 圆园员猿冤园远原0134-04 Research of correlation on auxin and expression of ARF in different tissues of poplar LI Xiao-yan, CHEN Xue-mei, GA I Y ing (College of Biological Sc ie nces and Tec hnology, Be ij ing Fores t Unive rsity, Beij ing 100083, China) Abstract: The biosynthesis of lignin is a complex process and many enzymes involve in the procedure. A RFs play a key role in the lignin biosynthsis which can affect gene transcription activation or repression by adj usting the early gene expression. This article mainly carriesd on the analysis of evolution and correlation of the available sequence and auxin based on A RFs gene chip data, the results showed that the conservation of poplar A RF sequence up to 60% . The relativity of up-regulated genes number, auxin content and signal of gene expression with each other was high in summer and autumn. The effect of winter on the semaphore of the auxin response was the largest. Key words: A RFs; poplar chip; auxin; correlation 生长素在植物的生长发育过程中袁 起着关键性的调 生长素含