球孢白僵菌bb174固态发酵产几丁质酶产酶及酶学-应用生态学报.pdfVIP

2004 5 15 5 CHINESE JOURNAL OF APPLIED ECOLOGY, May 2004, 15( 5)!863~ 866 Bb174 * 1 1 1 1 1 2* * 张 洁 蔡敬民 吴 克 金胜先 潘仁瑞 樊美珍 1 2 ( , 230022; , 230036) ( Beauveria bassiana) Bb174 . - 1 , 4!1! 1 g∀L , 75 g 3 ml - 1 ,pH 28 # 2 d, , 126 U∀g ( ) . 40 # , pH 50,30~ 70 # 1 h,48 # .30~ 40 # pH 4~ 6 , - 1 - 1 . Line eaverBurk ,K 052 mg∀ml , V 07 ∃ E ∀h . m m 680 1001- 9332(2004) 05- 0863- 04 Q9381 A Production and properties of chitinase from Beauver ia bassiana Bb174 in solid state fermentation. ZHANG 1 1 1 1 1 2 1 Jie ,CAI Jingmin , WU Ke , JIN Shengxian , PAN Renrui , FAN Meizhen ( Dep ar tment of Biology Science and Technology , H ef ei College, H ef ei 230022, China; 2 nstitute of Economic nsects and Fungi , A nhui A gr i cultur al University , H ef ei 230036, China) . Chin. J . App l . Ecol . ,2004, 15( 5) :863~ 866. This paper studied the chitinase production of Beauver ia bassiana Bb174 under solid state fermentation condi tion.The optimal medium consisted of heat bran and silk orm chrysalis at the ratio of 4!1,supplemented ith - 1 1 g peptone∀L as nitrogen source and some other mineral nutrients. The enzyme activity reached 126 units per gram dry medium after cultured for 2 days at 2