马蓝叶中葡萄糖苷酶的提取及酶学性质的研究.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 41 9 2010 9 ! 1461 ! 1 1 1* 2 刘泽玉 , 苏柘僮 , 杨 明 , 邹文铨 ( 1 , 610000; 2 , 610000) : pN PG , , pN PG 1 mL, 45 min, pNPG 02 mL , 25 mmo l/ L , pH 6, 55 , 30~ 60 pH 50~ 70 , , pH 4 , , pH : ; pN P G ; ; : R28602 : A : 2010) 09146 104 Extraction and enzymatic properties of glucosidase in leaves of Bap hica canthus cusia 1 1 1 2 L U Zey u , SU Zhet ong , YA NG M ing , Z OU Wenquan ( 1 Cheng du U niver sity of T r aditional Chinese M edicine, Chengdu 6 10000, China; 2 Sichuan U niversit y, Chengdu 6 10000, China) Abstract: Objective T o establish pNPG met hod for act ivity study of g lucosidase in Baphicacanthus cusia and invest igat e the basic enzym atic pr opert ies, t hen prov ide accordance f or furt her study on pro cess ing principle of IndigoNaturalis Methods pNPG Met hod w as adopt ed t o invest igat e t he o pt imum condi t ions f or activity study of glucosidase Results T he opt imum condit ions w ere as f ollow ing: the react ion volume w as 1 mL , the react ion t ime w as 45 min, t he volume of substrat e pNP G w as 02 mL , t he concen trat ion w as 25 mmo l/ L , the pH value of buffer solut ion w as 6, and t he react ion t emperature w as 55 Besides this, w e invest igat ed basic enzy matic pro pert ies of gluco sidase in B. cusia t indicated t hat gluco sidase w as unst able under 30- 60 , w hile it w as st able under 4 t w as st able under pH 50- 70 and it s act ivity decline rapidly acidic condi