温度和ph对南美自对虾主要消化酶活性的影响-华南农业大学学报.pdfVIP

第29 卷第4 期 华南农业大学学报 Vo l. 29 , No.4 2008 年 10 月 lournal of South China Agricultural University Oct. 2008 温度和 pH 对南美自对虾主要消化酶活性的影响 黄燕华，王国霞，刘襄河，周 哗，程军德 (珠海市农业科学研究中心，广东珠海 519070) 摘要:通过改变酶反应温度和 pH ，分析了南美自对虾蛋白酶、脂肪酶和淀粉酶活性的变化.结果表明，胃、肝膜脏、 肠道脂肪酶的最适温度分别为50 、30 、40 c ，淀粉酶的最适温度均为30 c ，蛋白酶的最适 pH 分别为 6.0 、6.0 ，7.5 ， 脂肪酶的最适 pH 均为 7.0 ，淀粉酶的最iè pH 分别为6.0 、 7.0、 6.5. 说明脂肪酶在中性时活力最高，淀粉酶在中性 偏酸性时活力最高，蛋白酶在胃和肝膜脏偏酸性、在肠道中性偏碱性时活力最高在一定温度和 pH 范围内，脂肪酶、 淀粉酶的活力均呈先上升后下降的趋势 关键词:南美白对虾;消化酶活性;温度; pH 中图分类号:S912 文献标识码:A 文章编号: 1001-411X(2008)04-0087-05 Effects of Temperature and pH on Activities of Digestive Enzymes in Penaeus vαnnamei HUANG Yan-hua , WANG Guo-xia , LIU Xiang-he , ZHOU Ye , Cheng Jun-de (Zhuhai Agriculture Research Center ,Zhuhai 519070 ,China) Abstract:The effects of different temperature and pH on activities of three digestive enzymes in Penαeus uαnnαmei were examined. In stomach , hepatopancreas and intestine , the optimal temperature for lipase were 50 ,30 ,40 C, respectively and for amylase were all 30 C. In stomach , hepatopancreas and intes- tine , the optimal pH for protease were 6. 0 ,6. 0 ,7. 0 , respectively at water temperature of 40 C, that for amylase were 6. 0 ,7. 0 ,6. 5 , and that for lipase were all 7. o. The results indicated that protease was con- ductive to weak acidic condition in stomach and hepatopancreas and to weak alkaline condition in intes- tine , lipase was conductive to neutral condition and amylase to acidic condition. The activities of lipase and amylase increased ,then decreased within the test temperature or pH range. K