液体活检专家共识.pptxVIP

液体活检的专家共识军事医学科学院附属医院秦海峰基因变异类别及分析技术需求液态活检的应用耐药机制的精准分析精准诊断----分子分型Content基因变异类别及分析技术需求液态活检的应用耐药机制的精准分析精准诊断----分子分型Content基因变异类别活化原癌基因(proto-oncogene)癌基因活化(oncogene)基因突变扩增或缺失易位融合表观遗传修饰 …失活抑癌基因(tumor suppressor gene, TSG)功能缺失表达异常其他基因 Modifying gene调节功能异常分子标志物种类及其对应的检测方法Bio-specimen variationDNA：mutation, translocation, CNV, MSIDeletionRNA:Single gene expressionGene expression profileProteinSingle protein Protein profilingEpigenetic:Methylation pattern…TechnologyDNASequencing, RT-PCRqPCR, aCGHFISH, CISHRNAqRT-PCRMicroarray techniquesTaqMan? gene expression arrayProteinIHC, ELISA, mass spectrometry