氯化锂对尾吊大鼠骨丢失的防护作用-第三军医大学学报.DOCVIP

氯化锂1，牛银波1，刘广兴3，王婷梅1，黄 勇1，梅其炳1,2* （710072西安西北工业大学生命科学院空间生物实验模拟技术国防重点学科实验室1；710072西安第四军医大学药学系药理教研室2；15163苏州中国科学院苏州生物医学工程技术研究所3摘要目的 研究GSK3特异性抑制剂氯化锂（lithium chloride LiCl）对尾吊大鼠骨密度的影响。方法 SD雄性大鼠18只，分为3组：正常对照组（Control）、尾吊对照组、尾吊LiCl组每组6只。尾吊LiCl组大鼠每日灌胃给予LiCl 20 mg/kg·d）。分别于第1天和第15天使用双能X线骨密度测量仪测定大鼠左股骨骨密度（bone mineral densityBMD）；第15天处死大鼠取左胫骨近心端进行组织包埋切片，HE染色，进行骨组织计量学测量，包括骨小梁面积百分率 (Tb.Ar% )、骨小梁数量 (Tb.N)、骨小梁平均厚度（Tb.Th）；对大鼠右股骨进行三点弯曲实验，测量骨力学指标。采用Western Blot观察骨组织中β-catenin蛋白的表达。结果 实验结束后，尾吊对照组和尾吊LiCl组大鼠BMD均低于正常对照组（P0.05），而尾吊对照组大鼠BMD明显低于尾吊LiCl组（P0.0）；骨组织计量学结果，尾吊对照组大鼠骨小梁平均厚度低于正常对照组（P0.05），骨小梁面积百分率、骨小梁数量低于正常对照组（P0.0）尾吊LiCl组大鼠均高于尾吊对照组(P0.05)；骨力学测试结果，尾吊对照组大鼠股骨弹性模量低于正常对照组（P0.05）刚性系数低于正常对照组（P0.0），而尾吊LiCl组大鼠股骨弹性模量高于尾吊对照组(P0.05)，刚性系数明显高于尾吊对照组(P0.01)。Western blot结果显示，尾吊对照组β-catenin蛋白表达量明显低于正常对照组，而尾吊LiCl组β-catenin蛋白表达量明显高于尾吊对照组。结论 尾部悬吊能够造成大鼠废用性骨丢失，而LiCl能有效地预防尾吊所导致的骨质疏松。失重模拟大鼠；氯化锂；骨密度；骨和骨组织；生物力学 中图法分类号R681.4 [文献标志码] A Protective effects of lithium chloride on bone loss in hindlimb unloading rats Cui Meng1, Niu Yinbo1, Liu Guangxing3, Wang Tingmei1, Huang Yong1, Mei Qibing1,2 (1Key Laboratory for Space Bioscience and Biotechnology, Faculty of Life Science, Northwest Polytechnical University, Xi’an 710072, China; 2Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, China; 3Suzhou, the Institute of Biomedical Engineering Technology of Chinese Academy of Sciences, Suzhou 215163 , China) [Abstract] Objective To investigate the effect of lithium chloride in the bone loss of hindlimb unloading rats. Methods Eighteen male SD rats were randomly divided into three groups: control group, hindlimb unloading (HU) group, HU + LiCl group. The HU+ LiCl group were intragastric administrated with LiCl (20mg/kg·d). To measure the bone density (BMD) of the rats’ left femurs in the first and fifteenth day by dual energy X-ray absorptiometry (DEXA), then, after operation, all rats were sacrificed. The left canna majors were assayed by histomor