协同利用卵泡抑素-中华细胞与干细胞杂志.pdfVIP

( ) 2012 11 2 4 Chin J Cell Stem Cell (Electronic Edition), Nov 2012,Vol.2, No.4 中华细胞与干细胞杂志电子版 年 月第 卷第 期 241 •实验研究• 协同利用卵泡抑素、骨形成蛋白和Wnt11 对 人骨髓间充质干细胞向心肌样细胞分化的影响 李占全 赵勇 付晶京 【摘要】目的探讨协同利用卵泡抑素、骨形成蛋白和Wnt11 对人骨髓间充质干细胞 向心肌细胞分化的影响。方法 培养的人骨髓间充质干细胞发育到二代后，分别加入卵泡 Wnt11 RT-PCR 抑素、骨形成蛋白和 ，用相差显微镜观察细胞的形态变化。收集细胞， 检查 NKx2.5 MEF2c 结果 6 Wnt11 和 基因表达的变化。 卵泡抑素、骨形成蛋白 和 诱导了人骨 髓间充质干细胞出现类似于心肌细胞的形态学变化及心肌细胞形成有关基因的表达。结 论 协同利用卵泡抑素、骨形成蛋白和Wnt11 可以诱导人骨髓间充质干细胞向心肌样细胞 方向分化。 【关键词】卵泡抑素；  骨形态发生蛋白类；  Wnt11 ；  骨髓间充质干细胞；  心 肌细胞 Effect of follistatin,BMP and Wnt11 on the differentiation of human MSC into cardiomyocytes LI Zhan-quan ZHAO Yong FU Jing-jing the Department of Cardiology,The , , . People’s Hospital of Liaoning Province, Shenyang 110016,China Corresponding author:ZHAO Yong, Email:yongzhao599@163.com Abstract Objective To investigate the effect of follistatin, bone morphogenetic protein 【 】 and Wnt11 on the differentiation of human mesenchymal stem cells (MSC) into cardiomyocyte. Methods Human bone marrow was collected and mononuclear cells were isolated. MSC from passage 2 were cultured in conditional mediums containing follistatin, bone morphogenetic protein and Wnt11 at 37 ℃in a humid 5 ﹪CO2 air atmosphere. Cardiac differentiation of MSC was assessed at different time points by morphology and with the specific markers of cardiomyocyte. Results The combined use of follistatin, bone morphogenetic protein and Wnt11 induced MSC to differentiate toward the cardiomyocytes as demonstrated by the morphology ob