微波预处理水酶法提取茶叶籽油工艺优化.pdfVIP

※工艺技术 食品科学 2012, Vol. 33, No. 06 87 陈德经 (陕西理工学院 陕西省资源生物重点实验室，陕西 汉中 723001) 研究微波处理茶叶籽仁，水酶法提取茶叶籽油的工艺条件。茶叶籽仁粉碎 60 目加6 倍质量的水，经过 800W 微波处理 10min，加入纤维素酶 1.5%、果胶酶2.0% 、蛋白酶0.25% ，采用pH4.5 、酶解温度45 ℃、酶解6h ， 离心萃取茶叶籽油。结果表明，微波预处理茶叶籽能够促进水酶法提取茶叶籽油，出油率达 27.9% 。 微波处理；水酶法；提取；茶叶籽油 Optimization of Microwave Pretreatment Followed by Aqueous Enzymatic Extraction of Tea Seed Oil CHEN De-jing (Bio-resources Key Laboratory of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723001, China) Abstract ：The present paper describes an investigation into the extraction of tea seed oil by microwave pretreatment followed by aqueous enzymatic extraction. Tea seeds were pulverized to 60 mesh and added to a 6-fold volume of deionized water before microwave treatment for 10 min at a power level of 800 W. Thereafter cellulase, pectinase and acid protease were added at respective doses of 1.5%, 2.0% and 0.25% for hydrolysis at pH 4.5 and 45 ℃ for 6 h. Finally centrifugation and extraction were sequentially conducted to obtain tea seed oil. Microwave pretreatment could promote the aqueous enzymatic extraction of tea seed oil, resulting in an extraction yield of 27.9%. Key words ：microwave pretreatment ；aqueous enzymatic method ；extraction ；tea seed oil 中图分类号：TS222.1 ；TS224.4 文献标识码：A 文章编号：1002-6630(2012)06-0087-05 中国是世界产茶大国，茶园总面积达2200 万亩(约 蛋白质及碳水化合物，且在提油过程中能很好除去一些 147 万多公顷) ，每年可产茶叶籽400 余万吨，可榨取茶 油料中的有毒或抗营养因子。因此，利用水酶法提取 叶籽油40 余万吨，相当于种植 50 万亩(约 3 万多公顷)油 植物油脂有着广泛的应用前景[7-8] [9] 。Sineiro 等 利用水酶 菜的产油量，经济和社会效益显著[ 1 ] 。成熟茶叶籽由 法提取葵花籽油，提油率较高。我国将此技术用于大 23% ～27% 种壳