MMP-2和CXCR4mRNA在人肾透明细胞癌中的表达及意义.pdfVIP

118 JModUrol，Vo1．19No．2Feb．2014 · 基础研 究 · 文章编号：1009—8291(2014)02—011803 MMP一2和 CXCR4mRNA在人肾透明细胞癌中的表达及意义 陈 强 ，李文贤 ，林晓杰 ，孙立江 ，隋爱华。 (1．青岛大学医学院附属医院泌尿外科 ，山东青岛 266003；2．青 岛大学医学院，山东青 岛 266021；3．青 岛大学医学院附属医 院中心实验室，山东青 岛 266003) Expression0fM M P一2andCXCR4andtheirclinicalsignificanceinhumanclearcell renalcellcarcinoma CHENQiang ，LIWen—xian ，LIN Xiao—jie，SUN Ii—jiang ，SUIAihua。 (1．DepartmentofUrology，AffiliatedHospitalofMedicalCollege，QingdaoUniversity，Qingdao266003； 2．MedicalCollegeQingdaoUniversity，Qingdao266021；3．CentralI．aboratory，AffiliatedHospitalofMedi— calCollege，QingdaoUniversity，Qingdao266003，China) ABSTRACT：Objective Toinvestigatetheexpressionsandcorrelationofmatrixmetalloproteinases一2(MMP-2)andCXC chemokinereceptor4 (CXCR4)mRNA inhumanclearcellrenalcellcarcinoma(ccRCC)tissues，andtostudytheirassociation withclinicopath0logicalcharacteristics．Methods TheexpressionsofMMP一2andCXCR4mRNA in 68casesoffreshccRCC tissuesand36casesofnormalrenaltissuesweredetectedbyreversetranscriptionpolymerasechain reaction (RT—PCR)．Re_ suits Thehalf—quantityvaluesofMMP-2andCXCR4mRNA inccRCC tissueswere0．848±0．367and0．939±0．336，which weresignificantlyhigherthanthoseinnormaltissues(t一7．375，8．860。PO．05)．Furthermore，inccRCC tissuestheexpres— sionsofMM P-2andCXCR4m RNA hadasignificantassociationwith clinicalstage，histologicalgradeand lymphnodemetasta— sis(t一2．169～ 3．852，P O．05)，whilenotwith genderandageofpatientsandthediameteroftumors(t一0．201～ 0．630，P 0．05)．M oreover，therewasapositivecorrelationbetween theexpression ofMM P-2andCXCR4mRNA inhuman ccRCC tis— sues(r一0．832，P0．05)．Conclusions TheseresultssuggestedthatMMP．2andCXCR4mightplayacooperativerolein thegrowth，invasion andmetastasisofccRCC．Theco—detectionofM M P-2andCXCR4couldbeconduciveto thediagnosisand evaluation ofccRCC． KEY WoRDS：clearcellrenalcellcarcinoma(ccRCC)；MMP．2；CXCR4；RT—PCR 摘要 ：目的 研