桑葚花色苷的提取及对人乳腺癌细胞株-第三军医大学学报.docVIP

桑葚花色苷的提取及对人乳腺癌细胞株MDA-MB-453生长的抑制 王 湛，常 徽，赵长君，糜漫天400038 重庆，第三军医大学营养与食品安全研究中心，重庆市营养与食品安全重点实验室400050 重庆，重庆理工大学药学与生物工程学院摘要提取桑葚花色苷，并观察其对人乳腺癌细胞株MDA-MB-453生长的抑制作用。 将桑葚冷冻干燥，冷冻干粉于常温下用70%浓度的乙醇以1﹕15的料液比超声提取1 h，提取液采用Amberlite? XAD7HP型树脂以 ml/min的吸附流速和 ml/min的洗脱流速在pH 3.0酸性环境下纯化，得到桑葚花色苷提取物。利用CCK-8法检测桑葚花色苷提取物对人乳腺癌细胞MDA-MB-453增殖活力的影响；以24只雌性BALB/c裸鼠建立MDA-MB-453细胞移植瘤模型，随机分为对照组、低剂量组、中剂量组和高剂量组4组，分别以0、50、100、200 mg/kg·d）的桑葚花色苷提取物膳食干预，检测其对肿瘤生长的影响。 获得花色苷平均含量为41.8%的桑葚花色苷提取物；CCK-8法检测结果表明，桑葚花色苷提取物可显著降低乳腺癌细胞活力，并显示剂量-效应关系；体内试验结果表明，高剂量组和中剂量组移植瘤体积和质量均显著低于对照组，表明桑葚花色苷提取物膳食干预可有效抑制移植瘤的生长。 桑葚花色苷提取物能够显著抑制人乳腺癌细胞株MDA-MB-453的生长。关键词桑葚花色苷；乳腺癌；移植瘤；MDA-MB-453Effects of anthocyanin extract of mulberry fruit on the growth of transplanted tumor of breast cancer MDA-MB-453 cells Wang Zhan1，Fu yujie1,2，Chang Hui1, Zhao Changjun1, Mi Mantian1 (Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, 400038 China1；School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China2) [Abstract] Objective Study on the extraction of anthocyanin compounds in mulberry fruit and observe its effects on the growth of breast cancer MDA-MB-453 cells in vitro and in vivo. Methods Dry powder of mulberry fruit after freeze dehydration was diffused by 70% ethanol with sonication, the material-liquid ratio was 1﹕15. The extracting solution was purified by Amberlite? XAD7HP resin in pH 3.0 acid environment, the absorption flow rate was 1 ml/min and the elution flow rate was 1 ml/min. Cells viability was detected by CCK-8 assay. 24 female BALB/c nude mice were injected with MDA-MB-453 cells to establish transplanted tumor models. The mice were equally randomized into 4 groups including control group, low dose, middle dose and high dose treatment groups. Oral administration of mulberry fruit anthocyanin extract (0, 50, 100, 200 mg/kg/day respectively) was given to the mice bearing cancer cell