安徽长江水系黄鳝的群体遗传结构分析-水产学报.PDFVIP

广东农业科学 2015 年第 1 期 113 安徽长江 系黄鳝的群体遗传结构分析 玉婷 江 河 王 潘庭双 段国庆 凌 俊 徽省农科院水产研究所袁 徽 合肥 230031冤 摘要 为研究安徽长江水系黄鳝 Monopte rus albus 冤的遗传多样性和群体遗传结构袁测定了其 6 个地理群体 当涂尧无为尧繁昌尧贵池尧怀宁和望江冤共 178 尾个体的线粒体 DNA 控制区部分序列遥对其长度为 556~558 bp 的控制 区同源序列进行分析袁共检测到变异位点 41 个 变异率 7.35%冤袁单倍型 56 种遥平均单倍型多样性和核苷酸多样性分 别为 0.694~0.954尧0.00237~0.01382遥群体分化指数 Fst 冤和基因流 Nm冤分别为 0.01974~0.87529尧0.07124~24.82928 袁分 子变异分析 A MOVA 冤中袁群体间遗传变异占 61.72% 袁表明黄鳝群体间具有明 的遗传分化遥基于单倍型或群体间遗 传距离的分子系统进化树均显示袁6 个地理群体分为两支院当涂与繁昌群体聚为一支袁其余 4 群体聚为另一支遥 关键词 黄鳝曰 控制区曰 长江曰 群体遗传结构 中图分类号 S917.4 曰Q347 文献标识码 A 文章编号 1004-874X 2015冤01-0113-06 Population genetic structure analysis of Monopterus albus from Anhui reaches in the Yangtze River HU Yu-ting, J IANG He, HU Wang, PAN Ting-shuang, DUAN Guo-qing, LING Jun (Fis he ry Re searc h Ins titute , A nhui A cade my of A gric ultural Sc ie nc es , Hefei 230031, China) Abstract: To assess the genetic diversity and population structure of Monopte rus albus, 178 indiv iduals of 6 populations (DT, WW, FC, GC, HN, WJ) from A nhui reaches in the Yangtze River were investigated based on 556-558 bp nucleotide sequences of mtDNA partial control region. The sequences analysis results showed that 41 variable sites (mutation rates of 7.35% ), 56 haplotypes were detected. The haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.694-0.954 and 0.00237-0.01382, respectively. A nalysis of molecular variance (A MOVA) revealed that genetic variation among the populations was 61.72% . T hese results indicated that there were significant genetic differentiations among populations. NJ -tree based on haploty pes or genetic d