基于柱切换技术的高压液相色谱法检测奶粉中的维生素D.PDFVIP

第4 卷 第2 期 食品安全质量检测学报 Vol. 4 No. 2 2013 年4 月 Journal of Food Safety and Quality Apr. , 2013 D * 周提军 , 王 莎, 李 兵, 荒井裕子, 城田修 (资生堂( 中国)投资有限公司先端科学事业推进部应用技术中心, 北京 100176) 摘 要: 目的 建立奶粉中维生素 D 的柱切换式高压液相色谱分析方法。方法 奶粉样品经过皂化处理、石 油醚提取、脱水浓缩后, 用柱切换式高压液相色谱仪进行检测。结果 维生素D3 在0.2~1.0 g/mL 范围内线性 关系良好, 回归方程 Y=1.74181e006X 0 相关系数 r=0.9956, 检出限为 0.01 g/mL, 定量限为 0.2 mg/kg, RSD 为2.12%, 平均回收率为 96.8% 。结论 该方法简单、快速、准确, 为复杂基质食品中低含量物质 的检测提供一定参考。 关键词: 阀切换; 维生素D; 高压液相色谱法 Detection of vitamin D in milk powder by HPLC based on column-switching system * ZHOU Ti-Jun , WANG Sha, LI Bing, HUANG JING Yu-Zi, CHENG Tian-Xiu (Advanced Science and Business Promontion Division Applied Technology Center of Shiseido (China) Investment Co ., Ltd ., Beijing 100176, China) ABSTRACT: Objective To establish a method for the determination of vitamin D in milk powder by high pressure chromatography based on column-switching methods. Methods After saponification treatment, milk powder was extracted with petroleum ether. The extract was dehydrated with anhydrous sodium sulfate, con- centrated, and analyzed by HPLC. Results The method showed a good linearity over the range of 0.2~1 μg/mL for vitamin D with r=0.9956. The linear equations was Y =1.74181e006X 0and the average recoveries were 96.8% with the relative standard deviations(RSDs)of 2.12%. The LOD and LOQ were 0.01