核桃枯枝病新病原及生物学特性-微生物学报.pdfVIP

微生物学通报 Jun. 20, 2017, 44(6): 1339−1348 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.160946 研究报告 核桃枯枝病新病原及生物学特性 1 1 1* 2 李星月 何朋俊 朱天辉 潘宗英 (1. 611130) (2. 408000) 摘 要：【目的】鉴定四川省广元市剑阁县核桃枯枝病的病原，为该病害的防治奠定基础。【方 法】随机采集不同发病阶段的枝干样品30 份，采用常规组织分离法分离纯化核桃枯枝病病原菌， 用柯赫氏法则对菌株进行致病性验证，结合形态学特征和分子生物学方法对病原菌进行鉴定，采 用菌落生长法进行生物学特性研究。【结果】共分离得到180 个菌株。致病性测定结果表明核桃 枯枝病的病原菌为拟茎点霉Phompsis capsici ，这是首次关于Phompsis capsici 导致核桃枯枝病在 我国发生和研究的报道。在PDA 平板上该病原菌菌丝为白色，能产生椭圆形和线形两种分生孢 子。病菌菌丝生长以PDA 培养基为最适。菌丝能有效利用多种碳、氮源，在供试的9 种碳、氮 源中，适宜生长的碳源为蔗糖和果糖，氮源为钼氨酸和苯丙氨酸。最适生长温度为25 °C ，最适 pH 为6.0−7.0。黑暗条件下菌丝生长最快。【结论】Phompsis capsici 最易从伤口侵入致病。 关键词：核桃枯枝病，Phompsis capsici ，生物学特性 Characterization of a new pathogen in walnut twig blight LI Xing-Yue1 HE Peng-Jun1 ZHU Tian-Hui1* PAN Zong-Ying2 (1. College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan 611130, China) (2. Chongqing Industry and Trade Polytechnic, Chongqing 408000, China) Abstract: [Objective] We isolated and identified walnut blight pathogen from diseased walnut growing in Jiange county, Guangyuan city, Sichuan province, to control the disease. [Methods] A total of 30 infested branch samples at different developing stages were randomly collected. Pathogen isolation and purification were conducted from walnut twigs tissue by normal tissue isolation method. To fulfill Koch’s postulates, pathogenicity test was done with the isolates. In addition, the pathogen was identified based o