百合黄瓜花叶病毒及其检测.PDFVIP

植物保护. 2006, 32(1): 73-76 百合黄瓜花叶病毒及其检测 1 2** 3 4 4 刘文洪 洪 健 陈集双 叶美琴 黄东峰 （1. 浙江中医学院，浙江杭州 310053 ；2. 浙江大学分析测试中心，浙江杭州310029 ； 3 浙江理工大学，浙江杭州310018 ；4 浙江丽水市农业局，浙江丽水323000 ） 摘 要 应用酶联免疫吸附法（ELISA ）检测了浙江丽水和杭州的东方百合、亚洲百合等栽培品种上黄瓜花叶病毒 （CMV ）侵染，64 个田间样品有26 个带毒，检出率为40.6% ；dsRNA 检测结果与已知CMV-FQS 株系的电泳条带 相同，但百合组织中 CMVdsRNA 含量较低；电镜观察受CMV 侵染的田间样品中大多含有线状病毒，复合侵染率 为 35.9%；寄主反应测定显示从百合植株上获得的 CMV 均不能通过汁液摩擦接种侵染昆诺藜、苋色藜、普通烟、 心叶烟等6 科10 种指示植物。 关键词 黄瓜花叶病毒；百合；ELISA ；dsRNA 检测 中图分类号 Q949.718 S436.8 Detection of Cucumber Mosaic Virus Infecting Lily in China LIU Wen-hong1 HONG Jian 2* CHEN Ji-shuang3 YE Mei-qing4 HUANG Dong-feng4 (1.Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China; 2. Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, China; 3. Zhejiang Univercity of Science, Hangzhou 3100 18, China; 4. Agriculture Bureau of Lishui, Lishui 323000, China ) Abstract CMV was detected from 26 out of 64 samples of lily from Lishui and Hangzhou, Zhejiang China, by enzyme-linked immunosorbent assay (ELISA). The rate of CMV was 40.6%. The result of dsRNA examination suggested CMV infecting lily have the same dsRNA patter with known CMV isolates obtained from tomato, but the content of dsRNA of in infecting lily tissue was relatively low. Virus particles of filamentous shape were detected from the above samples infected with CMV by electron microscopy, so the co-infection rate of filament virus and CMV was 35.9%. Host range tests suggested CMV obtained from lily couldn’t infect Nicotiana glutinosa ,N. tobacum ,Cucumis sativus, Chenopodium quinoa, C. amaranticolor and other tes